Mikrobiol. Z. 2022; 84(4):72-76.
doi: https://doi.org/10.15407/microbiolj84.04.072

Antimicrobial Activity of Bee Queen Larvae and Royal Jelly

B.P. Matseliukh1, А.V. Zakhariia2, H.I. Davydova2, S.M. Hotska2

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Acad. Zabolotny Str., Kyiv, 03143, Ukraine

2Prokopovych Institute of Beekeeping, NAAS of Ukraine
19 Akademik Zabolotny Str., Kyiv, 03143, Ukraine

Royal jelly has unique healing properties due to the presence of a number of biologically active compounds and yet unidentified components that are used for the prevention and treatment of many diseases. Purpose. To investigate the antimicrobial activity of homogenate of bee queen larvae and royal jelly against four phytopathogenic bacteria. Methods. Method of diffusion of biologically active compounds into agar medium sown with test cultures of phytopathogenic bacteria. Homogenates of bee queen larvae and royal jelly were diluted with sterile distilled water 1:5, centrifuged at 10,000 rpm to precipitate insoluble components, and 100 μL were added to the holes of the medium in Petri dishes seeded with phytopathogenic bacteria. After incubation of the dishes at 28ºC, the bactericidal effect of royal jelly was detected in the form of non-growth zones of phytobathogenic bacteria. Results. Royal jelly inhibited the growth of Clavibacter michiganensis subsp. michiganensis 102, Pectobacterium carotovorum 8982, Xanthomonas campestris pv. campestris 8003b, and Pseudomonas syringae pv. syringae 8511 around the holes in the medium. The largest zone of no bacterial growth (45.0 mm) was observed around the wells with royal jelly on the lawn of P. carotovorum 8982, which indicates the high sensitivity of these bacteria to an unidentified compound in royal jelly. Homogenate of bee queen larvae delayed the growth of only X. campestris pv. campestris 8003b. Thin layer chromatography revealed one similar and three different compounds in bee queen larvae and royal jelly. Conclusions. For the first time, the antibacterial activity of royal jelly against four phytopathogenic bacteria С. michiganensis subsp. michiganensis 102, P. carotovorum 8982, X. campestris pv. campestris 8003b, and P. syringae pv. syringae 8511 was established, which expands the spectrum of its antagonistic activity and can be used in the future for practical purposes.

Keywords: bee queen larvae, royal jelly, antibacterial activity, phytopathogenic bacteria.

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  1. Mardanly SG, Pomazanov VV, Kiseleva1 VA, Neskorodov YaB. Biological activity of the components of royal jelly and bee venom. Pharmacy & Pharmacology. 2018; 6(5):419—439. https://doi.org/10.19163/2307-9266-2018-6-5-419-439
  2. Gunina LM, Pashchenko OO, Davydova HI, Hots`ka SM. Assessment of safety and efficiency of application of innovative biologically active product «Vitalar» in athletes. In: Abstracts of II International Scientific Conference «Last achievements of biotechnology»; 2013 24—25 Oktober; Kyiv, 2013. p. 40.
  3. Bozhenko B, Gudz N, Pashchenko O, et al. Use of dietary pyphytocomposite supplement «Vitalar» in the complex treatment of children affected by the Chernobyl accident. In: Materials of international interdisciplines scientific practical conference «Topical issues of human health»; Uzhhorod, 2014. p. 36—43.
  4. McCleskey CS, Melampy RM. Bactericidal properties of royal jelly of the honeybee. Journal of Economic Entomology. 1939; 32(4):581—587. https://doi.org/10.1093/jee/32.4.581
  5. Krasikova VI. Bactericidal properties of brood food. Pchelovodstvo. 1955; 32(8):50—53.
  6. Blum MS, Novak AF, Taber S. 10-hydroxy-A2-decenoic acid, an antibiotic found in royal jelly. Science. 1959; 130(3373):452—453. https://doi.org/10.1126/science.130.3373.452
  7. National Center for Biotechnology Information. PubChem Compound Database CID=5280963. https://pubchem.ncbi.nih.gov/compound/5280963
  8. Marques CN, Morozov A, Planzos P, Zelaya HM. The fatty acid signalling molecule cis-2-decenoic acid increases metabolic activity and reverts per-sister cells to an antimicrobial-susceptible state. Appl Environ Microbiol. 2014; 80(22):6976—6991. https://doi.org/10.1128/AEM.01576-14
  9. Joana G Vicente , Eric B Holub. Xanthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops. Mol Plant Pathol. 2013; 14(1):2—18. https://doi.org/10.1111/j.1364-3703.2012.00833.x
  10. Nandi M, Macdonald J, Liu P, Weselowski B, Yuan Z-C. Clavibacter michiganensis ssp. michiganensis: bacterial canker of tomato, molecular interactions and disease management. Mol Plant Pathol. 2018; 19(8):2036—2050. https://doi.org/10.1111/mpp.12678
  11. Arnold DL, Preston GM. Pseudomonas syringae: enterprising epiphyte and stealthy parasite. Microbiology. 2019; 165(3):251—53. https://doi.org/10.1099/mic.0.000715