Mikrobiol. Z. 2021; 83(6):49-54.
Detection and Investigation of Some Properties of the Regulators of Antibiotic Biosynthesis
Produced by Streptomyces Strains S. sp. AN26 and S. sp. B35
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Acad. Zabolotny Str., Kyiv, 03143, Ukraine
The aim of this work was the isolation, purification and some properties investigation of two regulators of antibiotic biosynthesis of streptomycetes. Methods includes extraction of regulators from agar cultures and their concentration by vacuum rotary evaporator, thin layer chromatography and spectrophotometry. Results. Two strains of streptomycetes AN26 and B35 isolated from soils of different regions of Ukraine produce the regulators restoring the landomycin E biosynthesis and sporulation in mutant strain Streptomyces globispoprus 1912-B2. Both regulators were purified by thin layer chromatography and have the same Rf 0.69. Absorption curves of regulators were established by means of spectrophotometry. Maxima of absorption of regulators were 232.5 nm. The next study of the isolated regulators by means of NMR will give the possibility to elucidate their molecular structures. Conclusions. It is shown that two strains of streptomycetes isolated from the soils of Askania Nova and Brovary produce transcriptional regulators such as signaling molecules, which, like A-factor, restore the biosynthesis of antibiotics landomycin E and streptomycin in test strains S. globisporus 1912-B2 and S. griseis 1439, respectively. In terms of absorption maxima, they are similar and differ from similar indicators of known regulators of streptomycetes. It is possible that these compounds belong to new, not yet described signaling molecules, and the answer to this question will give future studies of their molecular structure by NMR spectroscopy.
Keywords: soil streptomycetes, S. globisporus 1912-B2, S. griseus 1439, transcriptional regulators.
Full text (PDF, in English)
- O’Brien J, Wright GD. An ecological perspective ofmicrobial secondary metabolism. Curr Opin Biotechnol. 2011; 22:552–558. https://doi.org/10.1016/j.copbio.2011.03.010
- Seipke RF, Kaltenpoth M, Hutchings MI. Streptomyces as symbionts: an emerging and widespread theme? FEMS Microbiol Rev. 2012; 36:862–876. https://doi.org/10.1111/j.1574-6976.2011.00313.x
- Bakker MG, Schlatter DC, Otto-Hanson L, et al. Diffuse symbioses: roles of plant-plant, plant-microbe and microbemicrobe interactions in structuring the soil microbiome. Mol Ecol. 2014; 23:1571–1583. https://doi.org/10.1111/mec.12571
- Hopwood D. Streptomyces in nature and medicine: the antibiotic markers. New York: Oxford University Press; 2007.
- Odomnker S, Addo K. Bacteria resistance to antibiotics: recent trends and challenges. Int J Biol Med Res. 2011; 2(4):1204–2010.
- Matselyukh B, Mohammadipanah F, Laatsch H, Rohr J, Efremenkova O, Khilya V. N-methylphenylalanyl-dehydrobutyrine diketopiperazine, an A-factor mimic that restores antibiotic biosynthesis and morphogenesis in Streptomyces globisporus 1912-B2 and Streptomyces griseus 1439. The Journal of Antibiotics. 2015; 68:9–14. https://doi.org/10.1038/ja.2014.86
- Anisova LN, et al. Regulators of the development in Streptomyces coelicolor A3(2). Izv Akad Nauk SSSR Biol. 1984; 98–107.
- Pliner SA, et al. Isolation and primary characteristics of A-factor. Bioorg Chim. 1975; 1:70–76.
- Niu G, Chater KF, Tian Y, Zhang J, Tan H. Specialised metabolites regulating antibiotic biosynthesis in Streptomyces spp. FEMS Microbiology Reviews. 2016; 40(4):554–573. https://doi.org/10.1093/femsre/fuw012
- Creamer KE, Kudo Y, Moore BS, Jensen PR. Phylogenetic analysis of the salinipostin γ-butyrolactone gene cluster uncovers new potential for bacterial signalling-molecule diversity. Microbial Genomics; 2021; 7:000568. https://doi.org/10.1099/mgen.0.000568
- Matselyukh BP, Lavrenchuk VY, Bambura OI. Screening and characteristic of regulators of antibiotic biosynthesis in Streptomyces. Mikrobiol Z. 2017; 79(2):95–102. https://doi.org/10.15407/microbiolj79.02.095