Mikrobiol. Z. 2021; 83(5):30-41.
Lelliottia nimipressuralis (Carter 1945) Brady et al. 2013 – the Causative Agent
of Bacterial Dropsy of Common Oak (Quercus robur L.) in Ukraine
I.M. Kulbanska1, M.V. Shvets2, А.F. Goychuk1, L.H. Biliavska4, V.P. Patyka3
1National University of Life and Environmental Sciences of Ukraine
19 General Rodimtsev Str., Kyiv, 03041, Ukraine
2Polissia National University
7 Staryi Blvd, Zhytomyr, 10008, Ukraine
3Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
4Poltava State Agrarian Academy
1/3 Skovoroda Str., Poltava, 36000, Ukraine
The article presents the symptomatic characteristics of vascular-parenchymal bacteriosis, which is quite common on forest woody plants, caused by the causative agent of bacterial dropsy. It was shown that a characteristic feature of this bacteriosis is the formation of various shapes and sizes of ulcers, in the places of formation of which there is a release of a dark, almost black, exudate. It is emphasized that during the study of the vascular system of common oak trees, its dark coloration is noticeable. Now there is a chronic course of the disease with the presence of dieback of individual branches in the crown. It was established that the causative agent of bacterial dropsy is Lelliottia nimipressuralis; its physiological and biochemical characteristics are given. The aim of the work is the bacteriological study of wood samples with typical signs of bacterial dropsy, study of morphological, cultural and biochemical properties of the causative agent of bacterial dropsy of oak. Methods. Classical microbiological, phytopathological, biochemical, statistical methods were uses in the work. If a bacterial dropsy was suspected, diagnostic methods were combined, with the help of which the etiology of the disease was established: an accurate analysis of symptoms; a thorough microscopic examination of the affected parts of plants; isolation and identification of the pathogen using NEFERMtest24 MikroLaTE, ErbaLachema, API 20E test systems and the like. Results. We have noted the habitual manifestations of the bacterial dropsy of the common oak. From the lesion, where dark brown areas of wood appeared under the bark in the area of the ulcer against the background of healthy light-colored wood, isolated bacterial isolates were identified by morphological and physiological-biochemical properties as Lelliottia nimipressuralis (Brady et al. 2013) – the causative agent of bacterial dropsy of oak (now Enterobacter nimipressuralis). It was found that isolates from common oak, like the collection strain E. nimipressuralis 8791, are gram-negative. It was shown that the studied bacteria are facultative anaerobes and use glucose both under aerobic and anaerobic conditions. They do not form gelatinase and protopectinase. Exhibit a positive Voges-Proskauer reaction. Bacteria use glucose, arabinose, rhamnose, amygdalin, and melibiose as the only source of carbon nourishment. The bacteria do not use inositol and sorbitol. They do not form indole and hydrogen sulfide (H2S), but are capable of reducing nitrates. They contain arginine dehydrolase, ornithine decarboxylase, β-galactosidase, but not lysine decarboxylase, and urease. Conclusions. It has been established that the diseases that we discovered are, by all symptomatic signs, a systemic, vascular-parenchymal bacteriosis, known as bacterial dropsy of oak, which affects all tissues, plant parts and generative organs at all stages of ontogenesis. According to the morphological, physiological and biochemical properties of bacterial dropsy isolated by us during the research, we identified the causative agent of bacterial edema of oak – Lelliottia nimipressuralis (Carter 1945).
Keywords: Lelliottia nimipressuralis, common oak, phytopathogenic bacteria and fungi, sudden death, etiology, pathogenesis, bacterial dropsy.
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- Press service of Volyn OULMG. The problem of mass deforestation was discussed at the board of the State Forest Agency. https://lisvolyn.gov.ua/?p=42381
- Oak drying is observed in Uzhhorod region. https://uzh-lishosp.com.ua/na-uzhhorodschyni-sposterihajetsya-vsyhannya-duba
- Volyn oaks give oak. https://www.openforest.org.ua/10489
- Oak Dropsy. https://fastivlis.net/no_cache/novini/novina/article/vodjanka-duba.html
- Kalinichenko NP. [Oak trees of Russia: monograph]. Moscow: VNIITslesresurs. 2000. 524–529. Russian.
- Molchanov AA. [The impact of anthropogenic factors on the forest]. Moscow: Nauka. 1978. Russian.
- Tsaralunga VV. [Cyclicity of accelerated withering away of oak]. Lesnoy vestnik. 2002; 2:31–35. Russian.
- Selochnik N, Oszako T, Delatour C. Oak decline in the forest-steppe region of Russia. Recent advances on oak health in Europe. Warsaw; 2000. p. 83–89.
- Vorontsov AI. [Forest pathology]. Moscow: Lesnaya promyshlennost. 1978. Russian.
- Borodayevskiy LS. [Drying out of the forest in the Mayatskaya dacha of the Maetsky forestry of the Kharkiv region]. Lesnoy zhurnal. 1909; 6:688–711. Russian.
- Polozhentsev PA, Savvin IM. [About the reasons for the withering away of oak forests]. Lesnoye khozyaystvo. 1976; 5:93–95. Russian.
- Kostin SI. [Influence of precipitation on the growth of early and late oak]. Lesovedeniye. 1968; 2:80–83. Russian.
- Izrail’skiy VP. [Bacterial plant diseases]. Moscow: Selkhozgiz. 1952. Russian.
- Cherpakov VV. [Etiology of bacterial dropsy of woody plants]. Izvestiya Sankt-Peterburgskoy lesotekhnicheskoy akademii. 2017; 220:125–139. Russian.
- Gvozdyak RI, Yakovleva LM. [Bacterial diseases of forest tree species]. Kyiv: Naukova Dumka; 1979. Ukrainian.
- Goychuk AF, Gordienko MI, Gordienko NM, et al. Patologiya dibrov. Kyiv; 2004. Ukrainian.
- Grünwald NJ, Goss EM, Press CM. Phytophthora ramorum: a pathogen with a remarkably wide host range causing sudden oak death on oaks and ramorum blight on woody ornamentals. Molecular Plant Pathology. 2008; 9(6):729–740. https://doi.org/10.1111/j.1364-3703.2008.00500.x
- Frankel SJ. Sudden oak death and Phytophthora ramorum in the USA: a management challenge. Australasian Plant Pathology. 2008; 37(1):19–25. https://doi.org/10.1071/AP07088
- Brasier C, Denman S, Brown A, Webber J. Sudden oak death (Phytophthora ramorum) discovered on trees in Europe. Mycological Research. 2004; 108:1108–1110. https://doi.org/10.1017/S0953756204221244
- Davidson J, Werres S, Garbelotto M, Hansen E, Rizzo D. Sudden Oak Death and Associated Disaeses Caused by Phytophthora ramorum. Plant Health Progress. 2003; 23. https://doi.org/10.1094/PHP-2003-0707-01-DG
- Tooley PW, Kyde KL. Susceptibility of Some Eastern Forest Species to Phytophthora ramorum. Plant Disease. 2007; 91(4):435–438. https://doi.org/10.1094/PDIS-91-4-0435
- Henry BW, Moses CS. An undescribed disease causing rapid dying of oak trees. Phytopathology. 1943; 33:18.
- Juzwik J, Harrington T, Macdonald W, Appel D. The Origin of Ceratocystis fagacearum, the Oak Wilt Fungus. Annual review of phytopathology. 2008; 46:13–26. https://doi.org/10.1146/annurev.phyto.45.062806.094406
- Shcherbyn-Parfenenko AL. [Cancer and vascular diseases of hardwood]. Moscow, Leningrad: Goslesbumizdat; 1953. Russian.
- Yachyevsikii AA. [Plant diseases: phytopathology]. Sanct-Petersburg; 1935. Russian.
- Goychuk AF, Drozda VF, Shvets MV, Kulbanska IM. Bacterial wetwood of silver birch (Betula pendula Roth): symptomology, etiology and pathogenesis. Folia Forestalia Polonica, Series A – Forestry. 2020; 62(3):145–159. https://doi.org/10.2478/ffp-2020-0015
- Goychuk A, Kulbanska I, Shvets M. Bacteria associated with Pseudomonas syringae pv. savastanoi in the pathology of Fraxinus excelsior L. Mikrobiol Z. 2020; 82(3):22–34. https://doi.org/10.15407/microbiolj82.03.022
- Klement E, Rudolph K, Sands S. Methods in phytobacteriology. Akademiai Kiado; 1990.
- Patyka VP, Pasichnyk LA, Dankevych LA, Moroz SM. Diagnosis of phytopathogenic bacteria. Guidelines. Kyiv; 2014. Ukrainian.
- Patyka VP, Pasichnyk LA, Gvozdyak RI, Petrichenko VF, et al. [Phytopathogenic bacteria. Methods of research. Monograph]. Vol. 2. Vinnytsia: Windruck; 2017. Ukrainian.
- Patyka VP, Pasichnyk LA, Butsenko LN, Petrichenko VF, et al. Express diagnostics of phytopathogenic bacteria and phytoplasmas in agrophytocenosis. Guidelines. Opole: Wydawnictwo I Drukarnia Ṡwiḙtego Krzyẑa; 2019.
- Brenner DJ, et al. Bergey’s Manual of Systematic Bacteriology. New York: Springer Science; 2005. https://doi.org/10.1007/0-387-29298-5
- Patyka VP, Pasichnyk LA. [Pathogenic bacteria: fundamental and applied aspects]. News of the Umansky National University of Gardening. 2014; 2:7–11. Ukrainian.
- Fuchylo Y, Pasichnyk L, Patyka V, Kalinichenko A. Bioenergy willow: protection from the negative impact of biological factors. In: Renewable energy sources theory and practict. Vol. II. Pietkun-Greber I, Ratuszny P, eds. Opole, Kyiv; 2017. p. 144–162.
- Alizadeh M. Bacterial Wetwood Disease. J Plant Chem and Ecophysiol. 2017; 2(1):1015. https://doi.org/10.26420/jplantchemandecophysiol.2017.1013
- Brady C, Cleenwerck I, Venter S, Coutinho T, De Vos P. Taxonomic evaluation of the genus Enterobacter based on multilocus sequence analysis (MLSA): proposal to reclassify E. nimipressuralis and E. amnigenus into Lelliottia gen. nov. as Lelliottia nimipressuralis comb. nov. and Lelliottia amnigena comb. nov., respectively. Syst Appl Microbiol. 2013; 36(5):309–319. https://doi.org/10.1016/j.syapm.2013.03.005
- Khodaygan P, Sedaghati E, Sherafati F. Isolation of Enterobacter nimipressuralis associated with bacterial wet wood from elm (Ulmus spp.) in Rafsanjan. Iranian Journal of Plant Pathology. 2011; 4(188):481–482.
- Liu S, Tang Y. Identification and Characterization of a New Enterobacter Onion Bulb Decay Caused by Lelliottia amnigena in China. Applied Microbiology. 2016; 2. https://doi.org/10.4172/2471-9315.1000114