Mikrobiol. Z. 2021; 83(1):3-11.
doi: https://doi.org/10.15407/microbiolj83.01.003

Efficiency of the Complex Bacterial Preparation Azogran Application in Protecting Potatoes from
the Colorado Potato Beetle Depending on the Stage of Its Development

I.K. Kurdish, A.A. Roy, I.A. Skoroсhod

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The Colorado potato beetle (Leptinotarsa decemlineana Say) is a particularly dangerous potato phytophage. The aim of this work was to study the effect of complex granulated bacterial preparation Azogran and its components on the spread of the Colorado potato beetle in the potato phytocenosis. Methods. The study was carried out during potato of the Slavyanka variety growing under the conditions of small-plot and laboratory experiments. The complex granulated preparation Azogran was made on the basis of interaction of nitrogen-fixing bacteria Azotobacter vinelandii IMV B-7076, phosphate-mobilizing bacteria Bacillus subtilis IMV B-7023 and clay mineral bentonite. 1 g of the preparation contains more than 108 viable bacteria of each strain. The mass of 1 granule was 0.25 g. The effect of Azogran, its components and 4-hydroxyphenylacetic acid (4-HPAA), a metabolite of B. subtilis IMV B-7023, on the survival of Colorado potato beetle larvae was estimated in laboratory conditions. Results. It was shown that the use of the complex bacterial preparation Azogran, B. subtilis IMV B-7023 strain and its metabolite 4-HPAA in the potato phytocenosis significantly reduces the spread of the Colorado potato beetle imago, as well as the number of egg-laying on the leaf surface of plants. Treatment of eggs clutches of the phytophage with this preparation and its specified components significantly reduces the number of larvae on the leaves. Treatment of 1–4 stages larvae with solutions of 4-HPAA at a concentration of 0.5–50.0 μg/ml, as well as a suspension of B. subtilis ІМV В-7023 bacteria or their culture medium (without cells), leads to significant death of the larvae. Conclusions. The use of the complex bacterial preparation Azogran, as well as its component bacteria B. subtilis IMV B-7023 in the phytocenosis of potatoes stimulates plant growth, significantly reduces the spread of the Colorado potato beetle and the number of eggs clutches on the leaf surface. Treatment of Colorado potato beetle larvae of 1–4 stages of development with a suspension of B. subtilis IMV B-7023 bacteria, as well as their metabolite 4-HPAA leads to the death of more than 65% of larvae. The results obtained indicate that the use of the complex bacterial preparation Azogran is promising for potatoes growing.

Keywords: complex bacterial preparation Azogran, potatoes, spread of the Colorado potato beetle and its larvae.

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