Mikrobiol. Z. 2020; 82(4):23-30.
doi: https://doi.org/10.15407/microbiolj82.04.023

Lipid Peroxidation Intensity in Different on Effectiveness of Symbiotic Systems Glycine max –
Bradyrhizobium japonicum under Drought Conditions

V.V. Morgun, S.Ya. Kots, T.P. Mamenko, N.A. Vorobey

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine

One of the mechanisms of reactive oxygen species action on plant tissues is the activation of lipid peroxidation, which can cause damage to cell membranes. It is known about the signaling functions of both reactive oxygen species and lipoperoxidation products, as well as the dependence of the intensity of oxidative processes on the ability of plants to realize their adaptive potential under stress. Aim. To study the intensity of lipid peroxidation processes in nodules, roots and leaves of soybean inoculated with strains and Tn5-mutants of Bradyrhizobium japonicum with different symbiotic properties under drought conditions. Methods. Microbiological, physiological, biochemical methods, gas chromatography and spectrophotometry. Results. It was found that effective symbiotic systems formed from the active strain Bradyrhizobium japonicum 646 and Tn5-mutant B1-20 are characterized by a slight increase in the content of malondialdehyde in nodules, roots and leaves during prolonged dehydration and quick restoration of its level to optimal after exposure to stress. For ineffective symbiotic systems formed with the participation of soybean and inactive Bradyrhizobium japonicum 604k strain, as well as inactive Tn5-mutant 107, significant development of lipoperoxidation processes under drought conditions was observed, which indicates a violation of cell membrane integrity. Conclusions. Activation of the lipid peroxidation process is a universal reaction of different symbiotic systems to the effect of drought, the intensity of which depends on their ability to realize their adaptive potential under unfavorable growing conditions.

Keywords: Bradyrhizobium japonicum, Glycine max (L.) Merr., malondialdehyde, lipid peroxidation, symbiotic systems, drought.

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