Mikrobiol. Z. 2020; 82(3):45-54. Ukrainian.
doi: https://doi.org/10.15407/microbiolj82.03.045

Fungicides Toxicity Assessment on Bradyrhizobium japonicum Nodule Bacteria in Pure Culture

N.А. Vorobey, K.P. Kukol, S.Ya. Kots

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine

Aim. To estimate the degree of Bradyrhizobium japonicum nodule bacteria strains sensitivity to systemic-contact fungicides Maxim XL, Standak Top, Fever, Akanto Plus and Benorad, which will help to resolve the issue of compatibility of plant protection chemicals with inoculation of seeds with bacterial preparations. Methods. Nodule bacteria sensitivity to the fungicides was studied by the method of wells in the agar medium plates with lawn seed nodule bacteria according to conventional microbiological methods. 80 μl of sterile tap water were placed into the center well of agar plate and used as control. Results. The studied B. japonicum nodule bacteria strains have different sensitivity to the single and double recommended norm of active substances of Standak Top, Fever, Akanto Plus and Maxim XL preparations with fungicidal effect. B. japonicum 634b, 646, 631, 71m, 48, 532C, 191, PC07, PC08, PC09, PC10, PC11 strains were evaluated as low-sensitive to single recommended dose of Benorad, however, the double dose of Benorad is toxic for most of the strains, except B. japonicum 71m, PC07, PC10 (low-sensitive). Conclusions. The artificial simulation of the effects of single and double recommended norm of Maxim XL, Standak Top, Fever, Akanto Plus and Benorad fungicides on nodule bacteria made it possible to determine their degree of resistance to these preparation and to identify the most resistant rhizobia in order to further use them in technology of soybean growing.

Keywords: nodule bacteria, Bradyrhizobium japonicum, fungicides, Maxim XL, Standack Top, Fever, Acanto Plus, Benorad.

Full text (PDF, in Ukrainian)

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