Mikrobiol. Z. 2020; 82(3):35-44. Ukrainian.
doi: https://doi.org/10.15407/microbiolj82.03.035

Influence of Biological Inducers on Antimicrobial, Antiadhesive Activity and
Biofilm Destruction by Nocardia vaccinii IMB V-7405 Surfactants

T.P. Pirog1,2, O.I. Skrotska1, T.A. Shevchuk2

1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

One of the effective approaches to increasing antimicrobial activity of secondary metabolites is the co-cultivation of the producer with competitive microorganisms. Aim. To study the antimicrobial, anti-adhesive activity, effect on the destruction of biofilms of Nocardia vaccinii IMV B-7405 surfactants synthesized in the presence of Escherichia coli IEM-1 and Bacillus subtilis BT-2 cells in a medium with industrial waste. Methods. Cultivation of N. vaccinii IMV B-7405 was carried out in a medium with refined and fried sunflower oil, as well as waste of biodiesel production. Live and inactivated by autoclaving E. coli IEM-1 and B. subtilis BT-2 cells were introduced into the medium at the beginning of the process and in the exponential growth phase. Surfactants were extracted from the supernatant of the culture liquid with a mixture of chloroform and methanol (2:1). The antimicrobial activity of surfactants was determined by the minimum inhibitory concentration, the effect of surfactant solutions on the adhesion of bacterial test cultures to polystyrene and the destruction of biofilms − by spectrophotometric method. Results. It was found that regardless of the moment of introducing competitive bacteria into the medium of producer cultivation and their physiological state, synthesis of surfactants was observed which antimicrobial activity against wide range of bacterial test cultures was 2–16 times higher, adhesion degree on polystyrene − 16–23% lower, and the degree of biofilms destruction – 10–35% higher compared with the parameters established for surfactants obtained on medium without competitive microorganisms. Conclusions. The introduction of live and inactivated bacterial inducer cells, in particular, E. coli IEM-1 and B. subtilis BT-2, into the medium with waste of biodiesel production and fried oil, allows to regulate not only antimicrobial, but also anti-adhesive activity of N. vaccinii IMV B-7405 surfactants and their ability to destroy biofilms.

Keywords: microbial surfactants, biological activity, competitive microorganisms.

Full text (PDF, in Ukrainian)

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