Mikrobiol. Z. 2020; 82(1):43-50.
Similarity of Genomic Sequences of Five Streptomyces globisporus Strains
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
The relevance of the research is that its results, firstly, will be useful in classifying streptomycetes to lower-order taxa, and secondly, can be used in studies on the evolution of organisms at the molecular level. Similar researches are widely conducted on housekeeping genes (for example, 16S rRNA). However, it is interesting to study successful applying in such researches of genes that determine not essential for survival proteins. The purposes of this research were to determine similarity of genomic sequences of 5 Streptomyces globisporus strains and to study whether it is possible to use the analysis of nucleotide sequences of genes encoding non-essential proteins or clusters of such genes (for example crt-genes) in determining the kinship of streptomycetes. Methods. Genomic sequences of 5 Streptomyces globisporus strains (C-1027, TFH56, NRRL B-2709, NRRL B-2293, and 1912-4Crt) were in NСBI databases. Computerized analysis of chromosomal DNAs sequences of streptomycetes were carried out by means of BLAST programs. Results. Genomic sequences of 5 S. globisporus strains were analyzed by BLAST program and similarity of lot of their characteristics were found. But many differences in sequences of 5 genomes were determined. It was found that the nucleotide sequences (of both the entire genome and its individual fragments) of S. globisporus NRRL B-2293 strain are the most different from the sequences of the other 4 chromosomes. The specific organization of its crt cluster can serve as a good example of such a distinction. We assume that it is necessary to revise the affiliation of NRRL B-2293 strain to the S. globisporus species. Conclusions. When performing classification (in addition to the traditionally used characteristics of genomes), we propose to analyze both non-essential genes and gene clusters – their presence in the genomes of streptomycetes, the level of similarity of nucleotide sequences of genes, and the organization of gene clusters.
Keywords: Streptomyces globisporus, crt-cluster, similarity of genomic sequences.
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