Mikrobiol. Z. 2020; 82(1):3-12.
doi: https://doi.org/10.15407/microbiolj82.01.003

The Functional Role of PPN1 and PPX1 Polyphosphatases under Stresses Action
and for Adaptive Response Development

S.I. Voychuk, E.N. Gromozova

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Enzymes of phosphorus metabolism play an important role in maintaining the life of cells of microorganisms under different environmental conditions. Two main polyphosphatases (poly(P)ases) PPN1 and PPX1 are known in the yeast cells. These enzymes participate in the polyphosphates metabolism and, thus, directly or indirectly involved in a number of intracellular processes. The purpose of our study was to study the genetic relationships between these poly(P)ases under stresses and their involvement in the process of an adaptive response in yeast cells. Methods. Strains of yeast defective by PPN1 and PPX1 poly(P)ases genes were used in the study. Genetic interaction was assessed by phenotypic traits using the fitness-test method. To establish the mechanism of participation of poly(P)ases in the processes of cellular response to the action of stresses, the activity of enzymes of the dehydrogenase complex was evaluated. The viability degree was used as an assessment of the adaptive response (AR) of yeast cells to the action of various stresses (acid, peroxide, hypertension). The induction of the AR in yeast cells was performed by means of exposure with the electromagnetic field (EMF) of the ultra-high frequency range (40.68 MHz). Results. It was shown that enzymes are involved in the work of different metabolic pathways and therefore regulate different aspects of cell activity. Both enzymes (PPN1 and PPX1) showed to be involved in the formation of the stress response by the yeast cells, however, only PPN1 had a direct impact on the formation of the AR since cells with a lack of this enzyme were unable to start AR as well. The deficiency on PPX1 stimulated the formation of AR in yeast cells even without EMF pretreatment, but its mechanism was different from the mechanism of AR induction under the action of EMF. The dehydrogenases showed to be important for the formation of cells response to the action of stress factors but did not play a direct role in the AR formation. Conclusion. These results expand the understanding of the poly(P) ases PPN1 and PPX1 functional role under stresses and for the AR formation.

Keywords: polyphosphatases, yeast, stress, fitness-test.

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