Mikrobiol. Z. 2019; 81(6):69-82. Ukrainian.
Immunomodulatory Effect of Probiotic Strain Lactobacillus casei IMV B-7280
on Physiological Norm in Experimental Animals
Lazarenko L.M., Babenko L.P., Spivak M.Ya.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
The aim of this work was to determine the immunomodulatory effect of the Lactobacillus casei B-7280 strain in the physiological norm by studying its effect on the functional activity of cells of the phagocytic system, the phenotypic composition of spleen lymphocytes and the level of pro- and anti-inflammatory cytokines in the blood serum of experimental animals. Methods. The B-7280 strain was administered to intact immunodeficient BALB/c mice orally using a probe or vaginally. Absorption activity, as well as oxygen-dependent bactericidal activity of macrophages of peritoneal exudate (MFPE) was assessed using common research methods. The number of CD16+/56+ natural killer cells (NKC), CD3+ T-lymphocytes, CD4+ T-helpers/inductors, CD8+ T-supressors/cytotoxic and CD19+ B-lymphocytes was determined in the spleen using the method of flow cytometry. The levels of pro- (interleukin (IL)-12 and interferon (IFN)-γ) and anti-inflammatory (IL-4) cytokines in serum were determined by enzyme immunoassay. Results. It was found that the B-7280 strain activated the phagocytic mechanisms of immune reactivity at the physiological norm: the number of MFPEs capable for absorbing the object of phagocytosis increased, the intensity of their absorbing function and digestive capacity increased and the level of functional reserve remained normal. The levels of IL-12 and IFN-γ in blood serum were increased in mice that received this strain both orally or vaginally, against the background of decrease or tendency to decrease the level of serum IL-4 in different terms of observation, which indicates its potential ability to induce the development of the immunity cellular link. At the physiological norm, the B-7280 strain did not influence the amount of NKC in the spleen and such important parameters of cellular and humoral factors of acquired immunity as the phenotypic composition of spleen lymphocytes. Activation of phagocytes and changes in the level of cytokines in mice’ blood serum after the use of strain B-7280 was not accompanied by deterioration of their physical condition or any form of intoxication. Conclusions. The B-7280 strain has immunomodulatory properties at the physiological norm aimed at activation of congenital immune factors and balancing the production of Th1/Th2 cytokines. The strain is promising for the development of probiotics (immunobiotics) with immunomodulatory properties, which can be used for the prevention and treatment of infectious and other diseases. The expediency of further studies of its biological activity and safety in different experimental models, as well as in clinical studies, has been substantiated.
Keywords: Lactobacillus, immune system, cytokines, physiological norm, experimental animals.
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