Mikrobiol. Z. 2019; 81(6):58-68.
doi: https://doi.org/10.15407/microbiolj81.06.058

Effect of Radiofrequency Electromagnetic Radiation on Photobacterium phosphoreum Luminescence

Hretskyi I.1,2, Zelena L.1, Gromozova E.1

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Kyiv National University of Technologies and Design
2 Nemyrovycha- Danchenko Str., Kyiv, 01011, Ukraine

The technological progress has led to the widespread use of various radiofrequency electromagnetic radiation (RF-EMR) sources. Luminous bacteria were used as test objects for research of radio waves influence on living organisms. Objective. The presented study was focused on the processes related to Photobacterium phosphoreum luminescence under RF-EMR: some physiological, biochemical consequences and the variations in luxb gene expression. Material and Methods. The IMV B-7071 strain of the luminous marine bacterium P. phosphoreum from the culture collection of the Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine was used as an object of the study. We used “UHF-62”, “Ray-11” and “MRTA-02” commercial devices as a source of RF-EMR. Results. It has been revealed that RF-EMR affected luminescence intensity, transcriptional activity of luciferase encoding gene expression, superoxide dismutase activity, cell survival rate. It was found that inhibition or stimulation of P. phosphoreum IMV B-7071 luminescence intensity depends on exposure duration. Conclusion. The data indicated the stressful nature of the RF-EMR action. Results obtained in this study suggest that luminescence intensity of P. phosphoreum IMV B-7071 bacterial cells is an indicator of the RF-EMR biotropic impact.

Keywords: bioluminescence, electromagnetic radiation, luxb gene expression, Photobacterium phosphoreum.

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