Mikrobiol. Z. 2019; 81(6):45-57. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.06.045

REP-PCR Analysis of Erwinia Genus Bacteria – Infectious Agents
of Apple Trees Diseases in Ukraine

Dankevych L.A., Muchnyk F.V., Patyka V.P.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. Genomes fingerprinting of isolated Erwinia sp., collection Erwinia horticola strains and the typical strain Erwinia amylovora UCM B-9057T was carried out for the correct species identification and evaluation of group heterogeneity. Methods. Microbiological, molecular genetic (REP-PCR) and statistical (UPGMA) methods were used in this research. Results. Тhe genetic heterogeneity of isolated Erwinia sp., collection E. horticola strains has been estimated. A significant relationship between isolated Erwinia sp. with typical E. amylovora UCM B-9057T strains for BOX, REP and ERIC profiles has been found. The heterogeneity of collection E. horticola strains by the given features has been determined. Conclusions. BOX, REP and ERIC profiling of bacteria of the Erwinia genus, infectious agents of apple tree diseases have been performed for the first time in Ukraine. The absence of genomic heterogeneity of fingerprints (100% of similarity) of isolated Erwinia sp. strains and their high similarity with fingerprint of typical E. amylovora UCМ В-9057Т strain have been determined. Similar profiles of the collection strains E. horticola are related to the profiles of E. amylovora UCM B-1095T by 85–92% (BOX-PCR), 78–89% (REP-PCR) and 64–100% (ERIC-PCR). Our results may be used for monitoring of distribution of these pathogens and identification of some strains as well as in population genetics, phylogenetics and biogeography research.

Keywords: identification, genetic heterogeneity, REP-PCR, Erwinia sp., Erwinia horticola, Erwinia amylovora.

Full text (PDF, in Ukrainian)

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