Mikrobiol. Z. 2019; 81(6):3-15. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.06.003

Effect of Complex Endophytic-Rhizobial Inoculation on Rhizosphere Microbiota
and Productivity of Soybean

Iutynska H.O.1, Goloborodko S.P.2, Tytova L.V.1, Dubinska O.D.2

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Institute of Irrigated Agriculture, NAAS of Ukraine
Naddnipryanske, Kherson, 73483, Ukraine

Modern studies of the microbiome of leguminous plants have revealed nodule bacteria and non-rhizobial prokaryotic endophytes, which inhabit the tissues of various organs. The interactions of symbiotic and endophytic bacteria, as well as their joint effect on the plant are not well studied. Аim. Тo study the rhizosphere microbiota of soybean and its productivity under complex inoculation by nodule bacteria together with non-rhizobial endophytic bacteria isolated from soybean nodules. Methods. Field experiments were conducted in the Kherson region under irrigation conditions in the fields of the Askanian Experimental Station of the Institute of Irrigated Agriculture of the National Academy of Agrarian Sciences of Ukraine. Seeds of soybean ultra-fast mature cultivar Diona and middle-mature cultivar Aratta were inoculated with RhizobinK bioformulation based on the association of nodule bacteria strains Bradyrhizobium japonicum UCM B-6018, B-6023 and B-6035, and also with complex of RhizobinK with B. megaterium B-5724, or with one of the endophytic bacteria strain: Paenibacillus sp.1, Bacillus cereus 4, Brevibacillus sp.5, Pseudomonas brassicacearum 6. The number of microorganisms of the main ecologicaltrophic groups in the rhizosphere of soybean was determined using the method of sowing the soil suspension on nutrient agar media. Results. The number of pedotrophic, phosphatemobilizing, oligoazotrophic and nitrogen-fixing bacteria was increased in the soybean rhizosphere soil of Diona and Aratta cultivars after inoculation with RhizobinK alone or together with one of the endophytes ‒ B. cereus 4, Brevibacillus sp.5, P. brassicacearum 6. Endophytic-rhizobial inoculation increases the number of beans and seeds on the plant. The highest yield was obtained after complex inoculation of soybean seeds of Diona and Aratta cultivars with RizobinK and B. cereus 4: 3.12 and 2.55 t/ha, respectively, which exceeded the parameters of the control variants by 26.6% and 16.5% respectively with simultaneous increasing of protein and fat in seeds. Conclusions. Research of complex effect of nodule and endophytic bacteria on plants will provide an opportunity to develop a strategy to increase the productivity of legumes based on new endophytic-rhizobial inoculants.

Keywords: rhizobia, endophytes, rhizosphere, soybean, seed productivity, yield quality.

Full text (PDF, in Ukrainian)

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