Mikrobiol. Z. 2019; 81(5):85-97.
doi: https://doi.org/10.15407/microbiolj81.05.085

Thermoactivation of Pseudomonas aeruginosa Pyocins

Balko O.I., Balko O.B., Avdeeva L.V.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The aim of work was the investigation of influence of different temperatures on activity of Pseudomonas aeruginosa S-type pyocins. Methods. The objects of investigation were S-type pyocins from eleven P. aeruginosa strains. These killer agents were subjected to the temperature influence in the range of 20 to 100°C for 10 minutes. Quantitative indices of killer activity of treated bacteriocins were compared with those ones of untreated analogs. For a more detailed study of 30 and 40°C effect on lysates, different durations of temperature treatment were used: 5, 15 and 25 minutes. Results. It was shown that 60°C caused the decrease in the activity of most bacteriocins to the minimum values, and 70°C – entailed the absolute loss of activity of all investigated lysates. Temperature treatment at 30 and 40°C increased the killer activity of bacteriocins. This effect was revealed with different rates depending on the used indicator cultures. The activity of the first group lysates increased after 30°С influence, the lysates from the second group – 40°С, and the third group – under the action of several temperatures, including 50°C or not changed. The 30°C effect on most bacteriocins stimulated 2-fold activity increase, and 40°C caused 4-fold rise in activity. But activity indices of some lysates extended more: 8 times – for РАЕ-24 and 16 times – for РАЕ-6. The investigation of influence of 30 and 40°C on PAE-19 and PAE-22 activity revealed no change in killer activity of bacteriocins caused by different duration of temperature impact. Conclusions. Temperature treatment of P. aeruginosa S-type pyocins at 30-40°С during 10 minutes makes possible to increase their activity in 2–4 times and in some cases, reach 8–16 times growth of activity indices. The extension of duration of temperature impact doesn’t stimulate the additional thermoactivation of bacteriocins.

Keywords: pyocins, thermoactivation, influence of temperature, increase of killer activity, Pseudomonas aeruginosa.

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