Mikrobiol. Z. 2019; 81(5):73-84. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.05.073

Antiadenoviral Activity of Titanium Dioxide Nanoparticles

Pankivska Yu.B.1, Biliavska L.O.1, Povnitsa O.Yu.1, Zagornyi M.M.2,
Ragulia A.V.2, Kharchuk M.S.1, Zagorodnya S.D.1

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Institute for Problems in Materials Science, NAS of Ukraine
3 Krzhizhanovsky Str., Kyiv, 03142, Ukraine

The analysis of non-toxic and environment-friendly substances and methods for the inactivation of infectious pathogens is one of the major areas of scientific research. In particular, it is known that titanium dioxide induces the production of active oxygencontaining radicals under the influence of UV radiation. As these reactive oxygen species can damage pathogenic biological molecules comprising proteins, lipids and nucleic acids, the compound is a promising agent for the development of preparations with virucidal and antiviral properties. The aim of the work. Investigation of the properties of titanium dioxide nanoparticles against adenovirus 5 serotype in vitro. Methods. The analysis of the porous structure of nanoparticles was carried out by adsorption-structural dynamic and static volumetric method. The structure of the test specimens was determined using an electron microscope JEM-1400 (JEOL, Japan). The cytotoxic, virucidal and antiviral effects of nanoparticles were determined using the MTT test. Results. Based on the analysis of antiadenoviral action of titanium dioxide nanoparticles, it was found that the nanopowder of the specimen (I) with particles of 8–15 nm in size was more active after a short incubation time (5 min) with adenovirus serotype 5, whereas a sample (II) with a particle size of 20–30 nm showed an inhibitory effect after 15 minutes of incubation. The antiviral activity of TiO2 (II) nanoparticles against human adenovirus 5 serotypes was between 45 and 95%. The use of the commercial nanopowder of TiO2 P25 produced by Evonik Industries AG (Germany) was found to be ineffective as the antiviral activity in the studied model system was absent. The most effective enhancement of the nanoparticles action of virucidal effect ТіО2 (ІІ) occurred at the concentration of 10 μg/ml and was approximately 30%. Conclusions. As a result of the research, it was found that titanium dioxide nanoparticles have a virucidal and antiviral effect against adenovirus serotype 5.

Keywords: adenovirus, nanoparticles, titanium dioxide, virucidal action, antiviral action.

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  1. Kavoosi F, Modaresi F, Sanaei M, Rezaei Z. Medical and dental applications of nanomedicines. APMIS. 2018; 126(10):795–803. https://doi.org/10.1111/apm.12890
  2. Toledano-Osorio M, Babu JP, Osorio R, Medina-Castillo AL, García-Godoy F, Toledano M. Modified Polymeric Nanoparticles Exert In Vitro Antimicrobial Activity Against Oral Bacteria. Materials (Basel). 2018; 11(6):1013. https://doi.org/10.3390/ma11061013
  3. Bowman MC, Ballard TE, Ackerson CJ, Feldheim DL, Margolis DM, Melander C. Inhibition of HIV Fusion with Multivalent Gold Nanoparticles. J Am Chem Soc. 2008; 130(22):6896–97. https://doi.org/10.1021/ja710321g
  4. Burdușel AC, Gherasim O, Grumezescu AM, Mogoanta L, Ficai A, Andronescu E. Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials (Basel). 2018; 8(9):681. https://doi.org/10.3390/nano8090681
  5. Zan L, Fa W, Peng T, Gong Z. Photocatalysis effect of nanometer TiO2 and TiO2-coated ceramic plate on Hepatitis B virus. J Photochem Photobiol. B: Biology. 2007; 86:165–69. https://doi.org/10.1016/j.jphotobiol.2006.09.002
  6. Park D, Shahbaz HM, Kim SH, Lee M, Lee W, Oh JW, et al. Inactivation efficiency and mechanism of UV-TiO2 photocatalysis against murine norovirus using a solidified agar matrix. Int J Food Microbiol. 2016; 238:256–264. https://doi.org/10.1016/j.ijfoodmicro.2016.09.025
  7. Mazurkova NA, Spitsyna YE, Zagrebel’nyi SN, Ryabchikova EI, Shikina NV, Ismagilov ZR. [Interaction of titanium dioxide nanoparticles with influenza virus]. Nanotechnologies in Russia. 2010; 5(5-6):417–20. Russian. https://doi.org/10.1134/S1995078010050174
  8. Kryukov AI, Stroyuk AL, Kuchmiy SYa, Pokhodenko VD. [Nanofotokataliz]. Kyiv: Akademperiodika; 2013. Russian.
  9. Zahornyi M. Nanosized powders as reinforcement for photoactive composites (Overview). Powder Metallurgy and Metal Ceramics. 2017; 56:130–147. https://doi.org/10.1007/s11106-017-9880-x
  10. HAdV_Working_Group, 2018. http://hadvwg.gmu.edu/
  11. Khanal S, Ghimire P, Dhamoon AS. The Repertoire of Adenovirus in Human Disease: The Innocuous to the Deadly. Biomedicines. 2018; 6(30). https://doi.org/10.3390/biomedicines6010030
  12. Lion T. Adenovirus infections in immunocompetent and immunocompromised patients. Clin. Microbiol. Rev. 2014;27(3):441–62. https://doi.org/10.1128/CMR.00116-13
  13. Farkas K, Marshall M, Cooper D, McDonald JE, Malham SK, Peters DE, et al. Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric viruses. Environ Sci Pollut Res Int. 2018. https://doi.org/10.1007/s11356-018-3261-y
  14. European Collection of Animal Cell Cultures Catalog. Porton Down: Salisbury (UK) PHLS Centre of Applied Microbiology and Research; 1990.
  15. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods.1983; 65(1-2):55–63. https://doi.org/10.1016/0022-1759(83)90303-4
  16. Kodama E, Shigeta S, Suzuki T, De Clerq E. Application of gastric cancer cell line (MKN-28) for anti-adenovirus screening using the MTT method. Antiviral Research.1996; 31:159–64. https://doi.org/10.1016/0166-3542(96)06966-5
  17. Carriel-Gomes MA, Kratz JM, Barracco MA, Bachére E, Monte Barardi CR, Oliveira Simões CM. In vitro antiviral activity of antimicrobial peptides against herpes simplex virus 1, adenovirus and rotavirus. Mem Inst Oswaldo Cruz, Rio de Janeiro, 2007; 102(4):469–72. https://doi.org/10.1590/S0074-02762007005000028
  18. Jukapli NM, Bagheri S. Recent developments on titania nanoparticle as photocatalytic cancer cells treatment. J Photochem Photobiol B. 2016; 163:421–30. https://doi.org/10.1016/j.jphotobiol.2016.08.046
  19. Kulkarni M, Mazare A, Gongadze E, Perutkova S, Kralj-Iglic V, Milosev I, et al. Titanium nanostructures for biomedical applications. Nanotechnology. 2015; 26(6):062002. https://doi.org/10.1088/0957-4484/26/6/062002
  20. Cho M, Chung H, Choi W, Yoon J. Different Inactivation Behaviors of MS-2 Phage and Escherichia coli in TiO2 Photocatalytic Disinfection. Appl Environ Microbiol. 2005; 71(1):270–75. https://doi.org/10.1128/AEM.71.1.270-275.2005
  21. Kato T, Tohma H, Miki O. Degradation of Norovirus in Sewage Treatment Water by Photocatalytic Ultraviolet Disinfection. Nippon Steel Technical Report. 2005; 92:41–44.