Mikrobiol. Z. 2019; 81(5):73-84. Ukrainian.
Antiadenoviral Activity of Titanium Dioxide Nanoparticles
Pankivska Yu.B.1, Biliavska L.O.1, Povnitsa O.Yu.1, Zagornyi M.M.2,
Ragulia A.V.2, Kharchuk M.S.1, Zagorodnya S.D.1
1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
2Institute for Problems in Materials Science, NAS of Ukraine
3 Krzhizhanovsky Str., Kyiv, 03142, Ukraine
The analysis of non-toxic and environment-friendly substances and methods for the inactivation of infectious pathogens is one of the major areas of scientific research. In particular, it is known that titanium dioxide induces the production of active oxygencontaining radicals under the influence of UV radiation. As these reactive oxygen species can damage pathogenic biological molecules comprising proteins, lipids and nucleic acids, the compound is a promising agent for the development of preparations with virucidal and antiviral properties. The aim of the work. Investigation of the properties of titanium dioxide nanoparticles against adenovirus 5 serotype in vitro. Methods. The analysis of the porous structure of nanoparticles was carried out by adsorption-structural dynamic and static volumetric method. The structure of the test specimens was determined using an electron microscope JEM-1400 (JEOL, Japan). The cytotoxic, virucidal and antiviral effects of nanoparticles were determined using the MTT test. Results. Based on the analysis of antiadenoviral action of titanium dioxide nanoparticles, it was found that the nanopowder of the specimen (I) with particles of 8–15 nm in size was more active after a short incubation time (5 min) with adenovirus serotype 5, whereas a sample (II) with a particle size of 20–30 nm showed an inhibitory effect after 15 minutes of incubation. The antiviral activity of TiO2 (II) nanoparticles against human adenovirus 5 serotypes was between 45 and 95%. The use of the commercial nanopowder of TiO2 P25 produced by Evonik Industries AG (Germany) was found to be ineffective as the antiviral activity in the studied model system was absent. The most effective enhancement of the nanoparticles action of virucidal effect ТіО2 (ІІ) occurred at the concentration of 10 μg/ml and was approximately 30%. Conclusions. As a result of the research, it was found that titanium dioxide nanoparticles have a virucidal and antiviral effect against adenovirus serotype 5.
Keywords: adenovirus, nanoparticles, titanium dioxide, virucidal action, antiviral action.
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