Mikrobiol. Z. 2019; 81(5):36-47. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.05.036

Correlation Dependence of the Antibiotic Compounds Biosynthesis and
other Biologically Active Substances in Soil Streptomycetes

Loboda M.I., Voichuk S.I., Biliavska L.A.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

It is known that soil streptomycetes are active producers of a wide range of biologically active substances, including antibiotics. Since the issue of the relationship between the antibiotics synthesis and the ways of other biologically active substances synthesis is not studied in full at present, the research subject is relevant. The question is not fully investigated. The goal of the work was to research the correlation dependence between the biosynthesis of antibiotic and other biologically active substances produced by soil streptomycetes. Methods. To determine the relationship between the synthesis of antibiotic compounds and other biologically active substances by soil streptomycetes was held factorial analysis with using the method of the main components; for identification the coefficients of regression and dispersion analysis were used the package Statistika v.10.0 (StatSoft. Inc., USA) and Mathlab v. 6.0; cluster analysis was held by using the Ward method with determination Euclidean distance as the degree of difference (in%) between the estimated variables. Results. Correlation relations between the antibiotic synthesis by strains Streptomyces violaceus IMV Ac-5027 and S. netropsis IMV Ac-5025 with biosynthetic pathways of other biologically active substances, but with different links are identificated. Synthesis of polyene and anthracycline antibiotics correlates with the synthesis of phytohormones (indole-3-carbinol, indole-3-carboxylic acid, isopentynyladenosine and gibberellins) and sterols (sitosterol). Production of these antibiotics also shows the dependence in the synthesis of triglycerides, sterols and their esters and fatty acids (isopalmitin, heptadecene, stearic fat acid, oleic fat acid, peanut fat acid). These antibiotics synthesis does not have a direct relationship with the content of amino acids. Conclusions. The relationships between biosynthesis of antibiotics, phytohormones and steroid compounds by soil streptomycetes S. netropsis IMV Ac-5025 and S. violaceus IMV Ac-5027 provides new tools for understanding the complex interconnections associated with the optimization of the synthesis of industrially important metabolites. Thus we can broad the knowledge about the conditions of their formation, the biosynthesis ways and functional significance for microbial cells.

Keywords: soil streptomycetes, antibiotics, metabolites, factorial and dispersion analyzes.

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