Mikrobiol. Z. 2019; 81(5):16-26. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.05.016

Viability of Azotobacter vinelandii IMB В-7076 and Bacillus subtilis IMB В-7023
in Nanocomposite of Bentonite

Кurdish I.K., Roy А.О., Voychuk S.I.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The elaboration of highly efficient forms of microbial preparations for plant production is an urgent task of science which will promote further biologization of agriculture and obtaining high quality products. Purpose. The investigation of the effect of the interaction between the strains of nitrogen-fixing bacteria Azotobacter vinelandii ІМV B-7076 and phosphate-mobilizing bacteria Bacillus subtilis IMV B-7023 with nanoparticles of natural clay mineral – bentonite, on the viability of cells. Methods. Nitrogen-fixing bacteria A. vinelandii IMV B-7076 were cultivated in the Ashby medium, and phosphate-mobilizing bacteria B. subtilis IMV B-7023 – in glucose-mineral medium. In manufactured 10–20% nanocomposite of bentonite 10% suspension of pure culture or the mixed bacterial culture were added. The viability of strains was determined after keeping them at room temperature during 1–6 мonths. Electronic microscopy of bacteria was conducted with the transmission electronic microscope JEM-1400 (Jeol, Japan). Results. The interaction between A. vinelandii ІМV B-7076 and B. subtilis ІМV В-7023 – components of a complex bacterial preparation and nanoparticles of 10–20% nanocomposite of bentonite enhanced the viability of these bacteria considerably. After six months of keeping them in 20% bentonite, the number of viable Azotobacter cells decreased by 45%, and of bacilli – by 11.5%, whereas in the medium without nanomaterials – by over 2 orders and 2.2 times respectively. The inoculation of wheat seeds with the complex nanocomposite bacterial preparation led to a considerable prolongation of the length of sprouts. Conclusions. The interaction between A. vinelandii ІМV В-7076 and B. subtilis ІМV В-7023 and nanobentonite enhanced the viability of these strains considerably, which is a prerequisite for manufacturing the complex nanocomposite bacterial preparation for plant growing using these bacteria.

Keywords: A. vinelandii ІМV В-7076, B. subtilis ІМV В-7023, viability of bacteria, nanocomposite of bentonite.

Full text (PDF, in Ukrainian)

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