Mikrobiol. Z. 2019; 81(4):107-117. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.04.107

Bioactivity of the Bacillus subtilis IMV B-7724 Extracellular Lectin

Fedosova N.I.1, Cheremshenko N.L.1, Getman K.I.2, Karaman O.M.1, Symchych T.V.1,
Ivanchenko A.V.1, Danyliuk O.I.1, Voeykova I.M.1, Didenko G.V.1

1Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
45 Vasylkivska Str., Kyiv, 03022, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To evaluate in vitro bioactivity of extracellular lectin of B. subtilis ІMV В-7724. Methods. The strain B. subtilis IMV B-7724 was used as a lectin producer. B. subtilis IMV B-7724 was cultured in stirred liquid Gause medium. Bioactivity (hemagglutinating, cytotoxic and cytolytic) of both the samples of the culture broth and lectin was studied in vitro. The statistical analysis was made using Student’s t-test. Results. The results indicate the presense in the B. subtilis ІMB B-7724 culture broth of lectins-like substanses with the pronounced cytotoxic and cytolytic effect on tumor cells. Lectin obtained on the 4th day of the culture growth had the highest hemagglutinating (2048 titer-1) and cytotoxic (IC=95.0±2.0%) activity. Conclusion. The significant hemagglutinating activity of substance isolated from the B. subtilis ІMB B-7724 culture broth indicates its belonging to the class of lectins. The extracellular bacterial lectin has significant cytotoxic activity in vitro against the experimental tumor cells.

Keywords: B. subtilis ІMV В-7724, culture broth, lectin, hemagglutinating activity, cytotoxic activity, cancer cells.

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