Mikrobiol. Z. 2019; 81(4):76-89. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.04.076

Biodegradation of Paracetomol by Nocardioform Actinobacteria

Khomenko L.A., Nogina T.M.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The aim of the work was to study paracetamol-degrading ability of actinobacteria and their growth using it as a substrate screen the strains of actinobacteria capable of dergrading paracetamol (N-(4-hydroxyphenyl)acetamide) and to study the characteristics of their growth on this indicated substrate. Methods. The work was performed using classical microbiological, physicochemical methods. The toxic effect of paracetamol was studied by serial dilution method. The concentration of paracetamol and its biotransformation products was determined by two methods - spectrometry and thin layer chromatography. Results. The ability of actinobacteria strains to biodegrade paracetamol has been studied. At the initial concentration of acetaminophen at 2 g/l actinobacteria have been shown to oxidize the drug in the medium to p-aminophenol, pyrocatechin, benzoquinone, hydroquinone and muconic acid, which are eco-friendly (non-toxic) compounds that exhibit positive effect on the growth and development of higher plants. After 5 days of cultivation the highest biodegradation activity of paracetamol (96.3%) was demonstrated by the strain Rhodococcus erythropolis UCM Ac-23. Сonclusions. The ability of actinobacteria to degrade acetaminophen indicates their potential for bioremediation of phenolic hydroxylcontaminated media.

Keywords: actinobacteria, paracetamol, biodegradation, drugs.

Full text (PDF, in Ukrainian)

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