Mikrobiol. Z. 2019; 81(4):62-75.
doi: https://doi.org/10.15407/microbiolj81.04.062

The Content of Hydrogen Peroxide and Catalase Activity in Different on Effectiveness of Symbiotic Systems
Glycine maxBradyrhizobium japonicum under Drought Conditions

Kots S.Ya., Mamenko T.P., Homenko Yu.A.

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine

Aim. To investigate the effect of prolonged drought on the changes in the content of hydrogen peroxide and catalase activity in the soybean roots and root nodules by inoculation Bradyrhizobium japonicum strains and Tn-5 mutants with contrasting symbiotic properties. Methods. Microbiological, physiological, biochemical, gas chromatography, spectrophotometry. Results. It has been established that effective symbiotic systems formed with the participation of soybeans and active strains of the B. japonicum 646 and Tn-5 mutant B1-20 differed for the manifestation of catalase activity and the content of hydrogen peroxide in the roots and root nodules during the effects of drought. However, these changes were accompanied by the development of stress – protective reactions and had an adaptive nature that led to the preservation of the effective functioning of their symbiotic apparatus in conditions of drought. In symbiotic systems formed with soybean and low-active Tn-5 mutant 107 and inactive strain B. japonicum 604k, increased hydrogen peroxide content and unstable activity of the enzyme under stress, as well as a poor recovery of their level to optimal in the post-stress period, were found. Conclusions. Efficiency of functioning of symbiotic systems Glycine maxB. japonicum for prolonged exposure to drought is marked by adaptive changes in the activity of catalase in the roots and root nodules that induce the regulation of the content of hydrogen peroxide and are accompanied by the preservation the work of symbiotic apparatus.

Keywords: Glycine max (L.) Merr., Bradyrhizobium japonicum, hydrogen peroxide, catalase, reactive oxygen species, drought.

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