Mikrobiol. Z. 2019; 81(4):15-28. Ukrainian.
doi: https://doi.org/10.15407/microbiolj81.04.015

Influence of Monovalent Cations on Synthesis and Biological Activity
of Nocardia vaccinii IMV B-7405 Surfactants

Pirog T.P.1,2, Kliuchka L.V.1, Shevchuk T.A.2, Iutynska G.O.2

1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. Study of the synthesis and biological properties of surfactants synthesized by Nocardia vaccinii IMV B-7405 in a medium with different content of potassium and sodium cations. Methods. Strain IMV B-7405 was grown in medium with glycerol. The surfactants were extracted from supernatant of cultural liquid by modified Folch mixture. Anti-adhesive activity and the degree of biofilms destruction were determined by the spectrophotometric method, antimicrobial activity – by index of the minimum inhibitory concentration (MIC). The activity of enzymes responsible for surfactants` biosynthesis was analyzed in cell-free extracts obtained after ultrasonic destruction of cells. Results. Addition of sodium chloride (0.5–1.0 g/l), potassium chloride or their mixture into the medium cultivation of the IMV B-7405 strain was accompanied by the synthesis of surfactants, the minimum inhibitory concentrations of which against bacteria and yeast were, respectively, in 2–125 and 2–30 times lower, the adhesion on abiotic surfaces treated with such surfactants was 1.1–1.6 times lower, and the degree of yeast biofilms destruction was 25–48% higher compared to the indicators established for surfactants obtained on the base medium. The antimicrobial and antiadhesive activity of surfactants correlated with NADP+-dependent glutamate dehydrogenase activity (a key enzyme of the aminolipids` biosynthesis, the most effective antimicrobial agents among surfactants). The increasing surfactants concentration in 1.2 times (up to 5.6 g/l) when strain IMV B-7405 was grown in the medium with 1.0 g/l of sodium chloride was associated with an increase in the synthesis of glycolipids, as evidenced by an increase of 1,6 times of phosphoenolpyruvate(PEP)-synthetase activity (a key enzyme of gluconeogenesis) compared with indicators on the base medium. Conclusions. The obtained data indicate the possibility of increasing the antimicrobial and anti-adhesive activity of microbial surfactants by changing in the cultivation medium the content of cations – potential activators of the key enzymes of biosynthesis components surfactants responsible for these biological properties.

Keywords: Nocardia vaccinii IMV B-7405, surfactants, potassium and sodium cations, antimicrobial and anti-adhesive properties, biosynthesis.

Full text (PDF, in Ukrainian)

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