Mikrobiol. Z. 2019; 81(2):25-35. Ukrainian.
Influence of Cultivation Conditions on Lysing Activity of Bacillus amiloliquefaciens Strain IMV-7571
Rybalchenko N.P., Kharkhota M.A., Avdeeva L.V.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. To investigate the influence of cultivation conditions on lysing activity of Bacillus amiloliquefaciens strain IMV B-7571. Methods. Lysing activity was determined by cocultivating the strain B. amiloliquefaciens IMV B-7571 and cyanobacterium Anabaena hassalii, Microcystis aeruginosa, M. pulverea in LB, NBY and glucose-mineral nutrient media. Determination of temperature and pH-optimum, study of the growth activity of strain B. amiloliguefaciens IMV B-7571 were studies by conventional methods. Results. The most lysis activity of strain B. amiloliguefaciens IMV B-7571 was at the 24th hour of its cultivation on LB medium. The level of lysis of test cultures cyanobacteria by the strain B. amiloliguefaciens IMV B-7571 was 54.76% for M. aeruginosa, 53.65% for M. pulverea and 58.33% for A. hassalii. The biological activity of strain B. amiloliguefaciens IMV B-7571 significantly depends on its cultivation temperature and pH. Lysing activity was recorded at a temperature of 35°C at pH 7.0. Conclusions. The obtained results will contribute to further research aimed at developing a biological biodrug with lysing activity based on strain B. amiloliguefaciens IMV B-7571.
Keywords: B. amyloliquefaciens, lysing activity, cyanobacteria.
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