Mikrobiol. Z. 2018; 80(6):79-93. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.06.079

Nitrogen-Fixing Activity of Soybean-Rhizobium Symbioses at the Complex Seed Inoculation

Kyrychenko O.V.

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine

Aims. Nitrogen-fixing activity of soybean-rhizobium symbioses at the mono- (Bradyrhizobium japonicum 634b) and complex (additional bioagents – Azotobacter chroococcum T79, phytolectins; lectin of soybean seed, wheat germ agglutinin) seed inoculation was studied in the greenhouse experiments with sandy and soil cultures at the using of acetylene reduction method. Results. It was shown that symbioses of soybean plants and complex inoculants characterized by increasing nitrogen-fixing activity of the symbiotic systems, functional activity of the one morpho-structural symbiotic unit (root nodule) as well as the nitrogen fixing ability of the rhizospheric microbiota in the early soybean development phases in the absence of root nodules by comparison to rhizobium monoinoculant. The different dynamics of the soybean-rhizobium symbioses nitrogenfixing activity at the complex seed inoculation was educed. Conclusion. Higher nitrogenfixing activity of its symbioses is a condition for ensuring a higher level of the plants nitrogen nutrition. As maximally perspective on the level of nitrogen-fixing activity intensity was marked the polyinoculant – rhizobium + (azotobacter + wheat lectin)іncubation. It was suggested to use the complex compositions for the soybean seed bacterization as one of the environmentally sound (biotechnological) ways of the increase of the symbiotic systems nitrogen-fixing activity.

Keywords: nitrogen-fixing activity, soybean-rhizobium symbioses, Rhizobium, Azotobacter, phytolectins, inoculants, intensity, dynamic.

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  1. Provorov NA, Shtark OY, Zhukov VA, Borisov AY, Tikhonovich IA. Developmental Genetics of Plant-Microbe Symbioses. New York: NOVA Science Publishers Inc.; 2010.
  2. Kots SYa, Morgun VV, Patyka VPh, Petrichenko VPh, Nadkernichnaia EV, Kirichenko EV. [Biological Nitrogen Fixation. Associative Nitrogen Fixation]. Kyiv: Logos; 2014. Russian.
  3. Isobe K, Ohte N. Ecological Perspectives on Microbes Involved in N-cycling. Microbes Environ. 2014; 29(1):4−16.
  4. Seefeldt L, Dance I, Dean D. Substrate Interactions with Nitrogenase: Fe versus Mo. Biochemistry. 2004; 43(6):1401−1409.
  5. Garipova SR. [The Ecological Role of Endophytic Bacteria in Symbiosis with Legumes and Their Use in Plant Breeding]. Advances in Current Biology. 2012; 132(5):493−505. Russian.
  6. Trivedi P, Pandey A, Palni LMS. Bacterial Inoculants for Field Applications under Mountain Ecosystem: Present Initiatives and Future Prospects. In: Maheshwari DK, editors. Bacteria in Agrobiology, Plant Probiotics. Berlin Heidelberg: Springer; 2012. p. 15−44.
  7. Kyrychenko OV. [Microbiological Aspects of the Regulation of Cultivated Plants Productivity and Protection]. Mykolaiv: Ilion; 2018. Russian.
  8. Kyrychenko OV. [Phytolectins and Diazotrophs are the Polyfunctional Components of the Complex Biological Compositions]. Biotechnologia Acta. 2014; 7(1):40−53. Ukrainian.
  9. Kandelinskaya OL, Grischenko ER, Ripinskaya KJu, Aleschenkova ZM, Kartizhova LE, Kuptsov VN, Kuptsov NS. [Role of Lectins in Regulation of Legume-Rhizobium Symbiosis Efficiency in Lupin]. Botanika (issledovaniya). 2015; (44):283−290. Russian.
  10. Pavlovskaya NE, Gagarina IN. [The Physiological Properties of Plant Lectins and Prerequisite for Their Application in Biotechnology]. Khimiya rastitelnogo syriya. 2017; (1):21−35. Russian.
  11. Bürgmann H, Meier S, Bunge M, Widmer F, Zeyer J. Effects of model root exudates on structure and activity of a soil diazotroph community. Environ Microbiol. 2005; 7(11):1711−1724.
  12. Dragovoz IV, Leonova NO, Biliavska LO, Yavorska VK, Iutynska GO. [Phytohormone Production by Some Free-living and Symbiotic Soil Microorganisms]. Dop NAS Ukr. 2010; (12):154−159. Ukrainian.
  13. Kao CM, Li SH, Chen YL, Chen SS. Utilization of the metano-cyan- complex tetracyanonickelate by Azotobacter vinelandii. Lett Appl Microbiol. 2005; 41(2):216−220.
  14. Antypchuk AF, Pilyashenko-Novokhatniy AI, Evdokimenko TM. [Practicum on Microbiology]. Kyiv: Manual University "Ukraine", 2011. Ukrainian.
  15. Hardy RWF, Burns RC, Holsten RD. Application of the Acetylene-Ethylene Assay for Measurement of Nitrogen Fixation. Soil Biol Biochem. 1973; 5(1):41−83.
  16. Krikunova TP. [Photosinteziruyuschaya Activnost Soyi v Zavisimosti ot Aktivnosti ee Rizobialnogo Kompleksa]. http://www.rusnauka.com/18_ADEN_2013/Biologia/9_141693.doc.htm Russian.
  17. Kizilova AK, Titova LV, Kravchenko IK, Iutinskaya GA. [Evaluation of the Diversity of Nitrogen-Fixing Bacteria in Soybean Rhizosphere by nifH Gene Analysis]. Microbiology. 2012; 81(5):621−629. Russian.
  18. Morgun VV, Kots SYa. [The Role of Biological Nitrogen in Nitrogen Nutrition of Plants]. Visnyk NAS Ukraine. 2018; (1):62−74. Ukrainian.
  19. Pat UA 62820 A, C05F11/08, C12N1/20 [Strain Bacteria of Azotobacter chroococcum T79 is for the Receipt of Bacterial Fertilizer under Soybean Plants]. Kots SYa, Tytova LV, Kyrychenko OV, Omelchuk SV, Zhemoyda AV. Opubl. 15.12.2003, Byul. N 12. Ukrainian.
  20. Tsavkelova EA, Klimova SYu, Cherdyntseva TA, Netrusov AI. [Microbial producers of Plant Growth Stimulators and Their Practical Use: A Review]. Rus J Appl Biochem Microbiol. 2006; 42(2):117−126. Russian.
  21. Tsavkelova EA, Klimova SYu, Cherdyntseva TA, Netrusov AI. [Hormones and Hormone-Like Substances of Microorganisms: A Review]. Rus J Appl Biochem Microbiol. 2006; 42(3):229−235. Russian.
  22. Sytnikov DM, Kots SYa, Malichenko SM, Kiriziy DA. [Photosynthetic Rate and Lectin Activity of Soybean Leaves after Inoculation with Rhizobia Together with Homologous Lectin]. Rus J Plant Physiology. 2006; 53(2):169−175. Russian.
  23. Kyrychenko OV, Volkogon MV. [Effect of Wheat Germ Agglutinin at the Presowing Treatment of Seeds on the Level of Cytokinins and Auxins in Leaves]. Dop NAS Ukr. 2010; (6):144−151. Ukrainian.