Mikrobiol. Z. 2018; 80(5):76-89. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.05.076

Influence of Fungicides on the Formation, Functioning and Peroxidase Activity
of Root Soybean Nodules at Inoculation by Rhizobia, Incubated with Lectin

Pavlyshche A.V., Mamenko T.P., Rybachenko L.I., Kots S.Ya.

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine

Aim. To study the influence of preparations with fungicidal action on the formation and functioning of a symbiotic apparatus and the reaction of protective enzymes with peroxidase activity (guaiacol- and ascorbate peroxidase) in root soybean nodules when inoculated with Bradyrhizobium japonicum, incubated of lectin. Methods. Microbiological, physiological, biochemical, gas chromatography, spectrophotometry. Results. The use of complex treatment of soybean seeds with fungicides (Fever, Maxime XL) and inoculant (B. japonicum 634b strain) induces an increase in the level of enzymes with peroxidase activity and contributes to the preservation of the effectiveness of the robots of the symbiotic apparatus. Soybean inoculation with a suspension of rhizobia incubated with lectin, with treatment of seeds with fungicides, had a negative effect on the formation and functioning of soybean - rhizobial symbiosis. Conclusion. Homologous lectin of soybean seeds, as a component of inoculation suppression, at a concentration of 100 μg/ml, raises the effectiveness of soybean symbiotic systems - Bradyrhizobium japonicum (strain standard 634b), however, can not be considered as an antidote for toxic effects of fungicides (Fever, Maxim XL, Standak Top) on a symbiotic apparatus in strictly controlled conditions of a model vegetation experiment and requires further research in soil culture.

Keywords: Glycine max (L.) Merr., Bradyrhizobium japonicum, guaiacol peroxidase, ascorbate peroxidase, symbiosis, fungicide, lectin.

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