Mikrobiol. Z. 2018; 80(5):25-35. Russian.
doi: https://doi.org/10.15407/microbiolj80.05.025

The Influence of Cultivation Parameters of Penicillium sp. 27 on the α-L-Rhamnosidase Activity

Gudzenko O.V., Borzova N.V., Varbanets L.D.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The aim. Study of the influence of some parameters of cultivation on the process of biosynthesis of extracellular α-L-rhamnosidase by the Penicillium sp. 27. Methods. Optimization of the growth medium was carried out on the Czapek base medium of the following composition, g/l: NaNO3 - 2; KH2PO4 - 1; KCl - 0.5; MgSO4×7H2O - 0.5; FeSO4×7H2O – 0,015; rhamnose - 4, pH - 6.0. As a carbon sources were used: xylose, arabinose, glucose, galactose, rhamnose, mannose, lactose, maltose, sucrose, mannitol. The sources of nitrogen were sodium nitrate, ammonium chloride, ammonium sulfate, ammonium acetate, yeast autolysate, yeast extract, peptone, urea, soybean flour. The α-L-rhamnosidase activity was determined by the Romero method, and the protein by the method of Lowry et al. Results. Investigation of the influence of some parameters of cultivation on the process of biosynthesis of α-L-rhamnosidase by Penicillium sp. 27 showed that for maximal biosynthesis the optimum sources of carbon and nitrogen were rhamnose (8 g/l) and peptone (3 g/l), temperature 25 °C , the initial pH of the medium was 5.0. It was established that the maximum level of α-L-rhamnosidase activity is reached on the seventh day of cultivation. Conclusions. When the Penicillium sp. 27 was grown under suitable conditions, the synthesis of α-L-rhamnosidase increased seven-fold. The activity of the enzyme in the supernatant of the culture liquid was 4.5 U/mg protein.

Keywords: α-L-rhamnosidase, parameters of cultivation, Penicillium sp. 27, sources of carbon and nitrogen.

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