Mikrobiol. Z. 2018; 80(5):15-24. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.05.015

Biodestruction of Hexahorcyclohexane Isomers by Natural and
Artificial Created Microbial Associations

Yamborko N.A.1, Iutynska G.А.1, Svistunova I.V.2

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2National University of Life and Environmental Sciences of Ukraine
13 Heroyiv Oborony Str., Kyiv, 03041, Ukraine

Aim. To study the specific destruction features of the HCH isomers complex by the artificially created association Biorem (Pseudomonas putida IMV B-7287, Bacillus megaterium IMV B-7287, Stenotrophomonas maltophilia IMV B-7288) and the native soil microbial association Micros, as well as individual strains – components of the association. Methods. Microbiological, gas chromatography, statistical. Results. It was found the Biorem association decomposes the HCH isomers complex in liquid medium to 70.9 - 86.7 % of initial level. S. maltophilia IMV B-7288 starts the destruction process the frst, the strains P. putida IMV B-7289 and B. megaterium IMV B-7287 are including the destruction process on the 4th - 7th day, therefore the active growth and destruction of HCH were prolonged. It was found that, depending on the conditions, one or another strain-destructor decomposed of HCH-isomers more intensively. So it is advisable to combine B. megaterium IMV B-7287, P. putida IMV B-7289 and S. maltophilia IMV B-7288 in the same microbial association (Biorem). Conclusions. Artificially created association of microorganisms-destructors Biorem can be applied as a bacterial preparation for remediation of HCH contaminated areas.

Keywords: microorganisms-destructors, organochlorine pesticides, HCH, lindane, biopreparation, biodegradation of contaminants.

Full text (PDF, in Ukrainian)

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