Mikrobiol. Z. 2018; 80(4):41-54. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.04.041

Serological Features of Bacteria Pseudomonas syringae Agroecosystems of Cereal

Pasichnyk L.A., Butsenko L.M.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To study the features of serogrouping of strains of Pseudomonas syringae pv. atrofaciens, isolated from wheat and segetal vegetation of the agroecosystem of wheat under various farming systems. Methods. Microbiological - the growth of bacteria on nutrient media, serological - the obtaining of O and OH antigens by the Grasse method, the agglutination reaction and double diffusion in agar. Results. According to the serological properties, the pathogenic strains of P. syringae pv. atrofaciens, isolated from wheat, belong to the four serological groups (II, IV, V, VI) according to the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas syringae group, developed by L.T. Pastushenko and I.D. Symonovych. In the population of the causative agent, strains of serogroup IV prevailed. Strains P. syringae pv. atrofaciens, isolated from the surface of apparently healthy wheat plants belonged to serogroup II. Isolated from weeds  strains of P. syringae pv. atrofaciens on antigenic composition belong to the five serological groups (I, II, IV, V, VI). Most strains belong to serogroup IV (53 %), a smaller number to serogroup II (22 %) and VI (13 %). Conclusions. It was established that strains of P. syringae pv. atrofaciens, isolated from wheat and weeds growing in wheat crops, are heterogeneous in antigenic composition. It is reveal a high degree of serological relationship of strains of P. syringae pv. atrofaciens, isolated from the segetal vegetation of wheat agroecosystems with the causative agent of basal bacteriosis of cereal crops. For the first time in the agrophytocenosis of wheat the strains of the causative agent that belong to serogroup I are found, which indicates a broader set of antigenic determinants in strains isolated from weeds.

Keywords: Pseudomonas syringae pv. atrofaciens, wheat, segetal vegetation, serological groups.

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