Mikrobiol. Z. 2018; 80(4):3-12.
Antimicrobial Activity of Some Essential Oils on Candida Genus Clinical Isolates
Kryvtsova M.V.1, Kohuch T.T.1, Salamon I.2, Spivak M.J.3
1Uzhhorod National University
32 Voloshina Str., Uzhhorod, 88000, Ukraine
2University of Presov
1 17th November Str., Presov, 08116, Slovakia
3Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aims. The purpose of the work was to study the qualitative composition of the concomitant opportunistic pathogenic microbiota in the patients suffering from pulmonary tuberculosis; analyze the sensitivity of the isolates to essential oils and antibiotics. Methods. For antibiotic susceptibility and antimicrobial activity testing was used disc diffusion method. The analysis of essential oils was carried out by Gas Chromatography method. Results. The study showed that microscopic Candida genus fungi were the dominating representative of the satellite microbiota isolated from the sputum – they were plated in 70 % cases. Most of the strains, even antibiotic-resistant ones, were ascertained to be susceptible to the essential oil of Thymus vulgaris L. The essential oils of Hyssopus officinalis L. and Rossmarinus officinalis L. were shown to be characterized by moderate antibacterial activity. The sensitivity to Mentha piperita L. and Savlia officinalis L. was strain-specific. Conclusion. By the level of antimycotic activity, the essential oils may be classified in a descending line beginning with Thymus vulgaris L. showing the most expressed antimicrobial activity, down to Hyssopus officinalis L., Rossmarinus officinalis L., Mentha piperita L., and Salvia officinalis L. The essential oil of Matricaria chamomilla L. showed no antimicrobial activity. The obtained results have proved the actuality of further studies of the impact of essential oils upon microorganisms, including those with multiple resistances to medical antibacterial preparations.
Keywords: Сandida strains, essential oils, antibiotic resistant microoganisms, antimycotic activity.
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