Mikrobiol. Z. 2018; 80(3):77-89. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.03.077

Activity of Superoxide Dismutase and Enzymes of Ascorbate – Glutathione Cycle in
Glycine maxBradyrhizobium japonicum Symbiotic Systems under Drought Conditions

Mamenko T.P., Homenko Yu.A., Kots S.Y.

Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine

Aim. To investigate the effect of drought on the activity of key antioxidant enzymes (superoxide dismutase, glutathione reductase, ascorbate peroxidase) in the soybean root nodules when inoculated with B. japonicum strains and Tn5 mutants with contrasting symbiotic properties. Methods. Microbiological, physiological, biochemical, gas chromatography, spectrophotometry. Results. Symbiotic systems, formed with the participation of soybean and the active strain B. japonicum 646, as well as the Tn5 mutant B1-20, are differed by adaptive changes in the activity of antioxidant enzymes under the action of dehydration. This is accompanied with saving of work effciency of their symbiotic apparatus. It is shown that in symbiotic systems formed with the participation of soybean and a low-activity Tn5 mutant 107, as well as an inactive strain of B. japonicum 604k, leads to differently directed changes of enzyme activity in nodules under the drought and weak recovery of their activity in the post-stress period. Conclusions. Formation of protective reactions of soybeans in symbiosis with B. japonicum under the actions of drought is associated with activation of superoxide dismutase, changes in the activity of enzymes of the ascorbate – glutathione cycle and depends from the ability of the symbiotic system to realize its adaptive potential under stress conditions.

Keywords: Bradyrhizobium japonicum, soybean (Glycine max (L.) Merr.), superoxide dismutase, glutathione reductase, ascorbate peroxidase, drought.

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