Mikrobiol. Z. 2018; 80(3):53-65. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.03.053

Genetic Profiling of Phytopathenic Bacteria of the Pseudomonas Genus – Agent
of Lupines Wet Watery Rot

Dankevych L.A., Patyka V.Ph.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. For correct species identification and estimation of group’s heterogeneity, the genome fingerprinting of isolated by us Pseudomonas sp. and collection “Pseudomonas xanthochlora” strains as well as typical representative of Pseudomonas marginalis species and five representative of Pseudomonas fluorescens species biovars has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR) methods and method of molecular phylogenetics (UPGMA) were used. Results. Genetic heterogeneity of isolated Pseudomonas sp. and collections “Pseudomonas xanthochlora” strains has been established. A significant relationship between isolated Pseudomonas sp. and collections “Pseudomonas xanthochlora” strains with the typical Pseudomonas marginalis pv. marginalis 9175T strain for BOX, REP and ERIC-profiles has been determined. Conclusions. BOX, ERIC and REP-profiling of the genome of the agent of lupines’ wet watery rot revealed no significant genetic heterogeneity of this group of strains (from 6 to 9 % of heterogeneity) and close its similarity to representatives of the species Pseudomonas marginalis (94 - 91 % of the homology of BOX, ERIC and REP-profiles) has been determined. The similarity of the results of genomes BOX, ERIC-profiling of the agent of lupines’ wet watery rot may be useful in the rapid correct diagnosis of this pathogen, especially, in case of pathogens spreading.

Keywords: identification, genetic heterogeneity, REP-PCR, Pseudomonas xanthochlora, Pseudomonas sp.

Full text (PDF, in Ukrainian)

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