Mikrobiol. Z. 2018; 80(3):29-39. Russian.
Polyphasic Taxonomic Analysis and Biologically Active Substances
of Strain Pseudomonas sp. 2303
Klochko V.V., Chugunova К.О., Avdeeva L.V.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Purpose. Determination of Pseudomonas sp. 2303 taxonomic status and study of its biological activity. Methods. Isolation of bacterial DNA, amplification and sequence of 16S rRNA gene, study of cultural, morphological, physiological and biochemical properties of strain and its antagonistic activity. Transmission electronic microscopy, GC/MS-analysis. Results. Phylogenetic analysis of 16S rRNA gene sequence of strain Pseudomonas sp. 2303 (1334 bp) in comparison with other genus Pseudomonas representatives established 100 % of identity with the type strain P. synxantha АТСС 9890Т gene and with the fluorescent species P. gessardii and P. libanensis. The latter differed from the studied strain in their phenotypic properties. The fatty acids analysis has shown that Pseudomonas sp. 2303 had in its composition characteristic for pseudomonads C16:0, C16:1, C18:1, 2OHC12:0 fatty acids сontent of which was similar with the type strain P. synxantha АТСС 9890Т. Strain Pseudomonas sp. 2303 had shown high activity against bacteria and fungi. Conclusions. According to polyphasic taxonomic analysis results the studied strain Pseudomonas sp. 2303 was identified as P. synxantha and included to Ukrainian Collection of Microorganisms as P. synxantha UCM В-399 (accession number in Genbank – MF196188). Some diagnostic characteristics were proposed (phenazine-1-carboxylic acid synthesis, inability of levan production from sucrose, assimilation of some carbon sources) which allow to differ P. synxantha from closely related species.
Keywords: polyphasic taxonomic analysis, Pseudomonas synxantha, P. gessardii, P. libanensis, fatty acids composition, antagonistic activity, phenazine-1-carboxylic acid synthesis.
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