Mikrobiol. Z. 2018; 80(3):15-28. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.03.015

Interaction of Bacteria with Solid Materials and Nanomaterials as Basis New Biotechnologies

Kurdish I.K.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The Department of Microbiological Processes on Solid Surfaces investigates the interactions of methanotrophic, nitrogen-fixing, phosphate-mobilizing and other groups of microorganisms with solid materials, including nanomaterials of various natures. The main objective of these studies is to determine the peculiarities of the effect of this processes on the physiological and biochemical activity of microorganisms and the development of the modern biotechnologies. For the first time it was proposed to use methanotrophic bacteria to reduce methane emissions from the worked out spaces of coal mines. Application of this biotechnology has allowed to reduce the methane release to the coal mining site by 47 – 60 %. It was established that the contact interaction of microorganism with nanoparticles of various nature increases their growth activity, cell viability through the influence of negative environmental factors and the result of spray drying. The technology of the increasing the yield of viable cells in bacterial preparation by the method of spray drying was introduced in the production of the preparation Litosil. The biotechnology preparation Azogran on the basis of the interaction of highly active strains of phosphate-mobilizing bacteria Bacillus subtilis IMV B-7023 and nitrogen-fixing bacteria Azotobacter vinelandii IMV B-7076 with nanomaterials of natural minerals use creation. It significantly improves the growth and dewelopment of ornamental, flower and other plants and increases the yield of technical, grain and vegetable crops by 18 – 37 %. Azogran preparation is introduced into production.

Keywords: methanotrophic bacteria, Bacillus subtilis, Azotobacter vinelandii, physiological and biochemical activity, nanomaterials, microbial biotechnology.

Full text (PDF, in Ukrainian)

  1. Kurdish IK, Myakenky VI, Demchenko VB, Kigel NF, Artemov KV, Trunov LF. [Prediction of intensity of microbiological methane oxidation in goafs of coal mines]. Microbiol. Z. 1992; 54(3):66-74. Russian.
  2. Gordienko AS, Kurdish IK. [Electrokinetic properties of Methylomonas rubra cells and their interaction with dispersed materials]. Microbiol. Z. 1990; 52(4):88-92. Russian.
  3. Kurdish IK, Kigel NF. [Effect of palygorskite, a clayey mineral, on physiological activity and adhesion of methanotrophic bacteria]. Microbiol. Z. 1992; 54(1):73-78. Russian.
  4. Gordienko AS, Zbanatskaya IV, Kurdish IK. Increase in the negative surface charge of Methylomonas rubra cells at contact interaction with the particles of silicon dioxide. Microbiol. Z. 1999; 61(5):48-55.
  5. Kurdish IK, Gordienko AS, Kigel NF and Kisten AG. Comparative Study on Adhesion of Various Species of Methanotrophic Bacteria to Solid Materials. Appl. Biochem. Microbiol. 1998; 34(3):222-226.
  6. Kisten AG, Kigel NF, Kurdish IK, Gordienko AS. [Effect of some physico-chemical environment factors on aghesion of methanotrophic bacteria]. Microbiol. Z. 1996; 58(3):62-70. Russian.
  7. Kisten AG, Roi AA, Kurdish IK, Shevchenko TV. Adhesion of Mixed Cultures of Methanotrophic Bacteria to Solid Materials. Appl. Biochem. Microbiol. 1998; 34(4):377-381.
  8. Gordienko AS, Shevchenko TV, Kurdish IK. [Effect of chemical modification of Methylocystis parvus cells surface on electrokinetic properties and adhesion of these bacteria]. Microbiol. Z. 1997; 59(2):24-29. Russian.
  9. Kurdish IK, Muchnik FV, Malashenko YuR, Dementieva OA. Specific [Concentration of Nutrients as a Criterion Controlling Microorganism Cultivation]. Mikrobiol. Z. 1988; 50(3):13-16. Russian.
  10. Kurdish IK, Muchnik FV, Malashenko JR. The mathematical model to control the microbial cultivation process by the specostat principle. Progress in Biotechnology. (Eds. A. Blazej, A.Ottova). Elsevier. Amsterdam-Oxford-New York-Tokyo. 1990; (6):175-182.
  11. Kurdish IK, Kravchuk VN, Egorov OV, Karpenko VI, Malashenko YuR. [Adhesion of methane-oxidizing bacteria to rocks of coal mines]. Microbiol. Z. 1990; 52(1):73-77. Russian.
  12. Kurdish IK, Kigel NF, Egorov OV. [Physiological activity of methanotrophic bacteria under their interaction with mountain rocks]. Microbiol.Z. 1991; 53(1):92-98. Russian.
  13. Kurdish IK. [On the problem of perspectiveness of application of certain species of methanotrophs to decrease metane liberation in coal mines]. Microbiol. Z. 1996; 58(4):86-92. Russian.
  14. Myakenky VI, Kurdish IK [Microbiological oxidation of coal mine methane]. Kiyv: Naukova dumka, 1991. Russian.
  15. A.C.USSR 166739. [Method of degassing the worked out lava space]. Myakenky VI, Kurdish IK, Demchenko V B and other. 1991; Bul. 45. Russian.
  16. Myakenky VI, Kurdish IK, Demchenko VB, Petukh AP, Shmigol AV, Trunov LF. [Efficiency of microbiological oxidation of methane in the exhausted spaces of coal mines]. Microbiol. Z. 1992; 54(1):67-73. Russian.
  17. Gordienko AS, Zbanatskaja IV, Kurdish IK. Change in electrosurface properties of Methylomonas rubra cells at contact interaction with particles of silicon dioxide. Can. J. Microbiol. 1993; 39(9):902-905. https://doi.org/10.1139/m93-136
  18. Kurdish IK, Kigel NF. [Effect of high-dispersed materials on physiological activity of methanotrophic bacteria]. Microbiol. Z. 1997; 59(2): 29-36. Russian.
  19. Kurdish IK, Bikhunov VL, Tsimberg EA, Elchits SV, Vygovskaya EL, Chuiko AA. [Effect of dispersed silicon dioxide-aerosil A-300 on the growth of yeast Saccharomyces cerevisiae]. Microbiol. Z. 1991; 53(2):41-44. Russian.
  20. Tsimberg EA, Titova LV, Kurdish IK. [Effect of highly-dispersed materials on the growth of Candida yeast]. Microbiol. Z. 1991; 53(4): 55-58. Russian.
  21. Kurdish IK, Titova LV, Tsimberg EA, Antipchuk AF, Tantsyurenko EV. [Effect of Aerosil on the Growth of Azotobacter chroococcum]. Microbiol. Z. 1993; 55(1):38-42. Russian.
  22. Titova LV, Antipchuk AF, Kurdish IK, Skochinskaya NN, Tantsyurenko EV. [Effect of high-dispersed materials on physiological activity of Azotobacter bacteria]. Microbiol. Z. 1994; 56(3):60-65. Russian.
  23. Kurdish IK,Titova LV. Use of High-Dispersed Materials for Culturing and Obtaining Granular Agrobacterium radiobacter Preparation. Appl. Biochem. Microbiol. 2001; 37(3):318-321. https://doi.org/10.1023/A:1010201806576
  24. Kurdish IK, Kigel NF, Bortnik SF. [Stabilization of physiological activity of methanotroph Methylomonas rubra 15 under storage]. Microbiol. Z. 1993; 55(4):37-43. Russian.
  25. Kurdish IK, Roy AA, Garagulya AD, Kiprianova EA. Survival and antagonistic activity of Pseudomonas aureofaciens UKM B-111 stored in fine materials. Microbiology. 1999; 68(3):387-391.
  26. Kurdish IK, Antonyuk TS. [Effect of clayey minerals on viability of some bacteria at high temperatures]. Microbiol. Z. 1999; 61(3):3-8. Russian.
  27. Kurdish IK, Titova LV. Granular Preparations of Azotobacter Containing Clay Minerals. Appl. Biochem. Microbiol. 2000; 36(4):418-420. https://doi.org/10.1007/BF02738054
  28. Kurdish IK. [Granulated Microbial Preparation for Plant-growing: Science and Practice]. Kyiv. 2001. KVITs. Russian.
  29. Gordienko AS, Kurdish IK, Krasnobrizhy NYa. [Effect of clay mineral palygorskite on survivability of bacteria under their dewatering]. Microbiol. Z. 1990; 52(5):75-78. Russian.
  30. Patent of Ukraine 14654A. [Method for obtaining dry bacterial preparation]. Kurdish IK, Krasnobrizhy NYa, Gordienko AS. Published 1991. Bul. 5. Ukrainian.
  31. Patent of Ukraine 54923A. [Strain of bacteria Bacillus subtilis for bacterial fertilizer obtaining for plant-growing]. Kurdish IK, Roy AO. Published in 2003, bulletin 3. Ukrainian.
  32. Patent of Ukraine 72856. [Strain of bacteria Azotobacter vinelandii for bacterial fertilizer obtaining for plant-growing]. Kurdish IK, Bega ZT. Published in 2006, bulletin 8. Ukrainian.
  33. Kurdish IK, Bega ZT. Effect of Argillaceous Minerals on Growth of Phosphatemobilising Bacteria Bacillus subtilis. Appl. Biochem. Microbiol. 2006; 42(4):388-391. https://doi.org/10.1134/S0003683806040089
  34. Chobotarjov AYu, Gordienko AS, Kurdish IK. [Influence of natural minerals on growth of Azotobacter vinelandii IMV B-7076]. Microbiol. Z. 2010; 72(5):27-31. Ukrainian.
  35. Gerasymenko IO, Kurdish IK. [Influence of Vermiculite and Silicon Dioxide on Dehydrogenase Activity of Bacillus subtilis IMV B-7023 and Azotobacter vinelandii IMV B-7076]. Microbiol. Z. 2015; 77(1):20-24. Ukrainian. https://doi.org/10.15407/microbiolj77.01.009
  36. Kurdish IK, Gerasimenko IO. [Influence of Physico-chemical Factors on Dehydrogenase activity of Azotobacter vinelandii IMV B-7076]. Microbiol. Z. 2017; 79(4):3-11. Ukrainian. https://doi.org/10.15407/microbiolj79.04.003
  37. Tsercovniak LS, Roy AA, Kurdish IK. [Synthesis of aminoacids of Bacillus subtilis IMV B-7023 in the medium with glycerophosphates]. Microbiol. Z. 2009; 71(5):18-23. Ukrainian.
  38. Roy AA, Gordienko AS, Kurdish IK. [Some properties of the patern and resistant to streptomycin strain Bacillus subtilis IMV B-7023 a compound of bacterial preparations for plant growing]. Current state and prospects for the development of microbiology and biotechnology. Minsk, June 1-2. 2006. Minsk, Rakov. 2006; 294-296. Russian.
  39. Kurdish IK, Roy AO, Skorochod IO, Chobotarov AYu, Herasimenko IO. [Elaboration of a free-flowing complex bacterial preparation for cereal crops]. Nanosize system and nanomaterials: studies in Ukraine. Kyiv, Akademperiodika, 2014; p. 610-614. Russian.
  40. Kurdish IK. [Influence of biogenic and abiogenic factors on the efficiency of microorganisms introduction into agroecosystems]. Microbiol. Biotech. 2009; (1):22-38. Ukrainian.
  41. Tserkovniak LS, Kurdish IK. Phosphate-mobilizing Bacteria Bacillus subtilis as Phenolic Producers. Appl. Biochem. Microbil. 2009; 45(3):279-284. https://doi.org/10.1134/S0003683809030077
  42. Ocheretyanko A, Skorochod T, Roy A, Kurdish I. Accumulation of Phenolic Compounds in Cultural Media of Phosphate-Mobilizing Bacteria of Genus Bacillus Cohn. Intern. J. Sci. Res. Knowledge. 2015; 3(5):131-138. https://doi.org/10.12983/ijsrk-2015-p0131-0138
  43. Ocheretyanko A, Skorochod I, Roy A, Kurdish I. Influence of Physical and Chemical Factors on the Accumulation of Phenolic Compounds Nitrogen-Fixing Bacteria Azotobacter vinelandii IMV B-7023. Acta Velit. 2016; 2(1):49-55.
  44. Brusnika VYu, Ocheretyanko AA, Useinov RSh et al. Influence of Azotobacter vinelandii IMV B-7076 on the content of phenolic compounds in cucumber plants. Quarantine and integrated plant protection. Prospects of development in the XXI century. Kyiv. 2015; p. 32-33.
  45. Kurdish I, Roy A, Skorochod I. et al. Free-Flowing Complex Bacterial Preparation for Crop and Efficiency of its use in Agroecosystems. J. Microbil., Biotechnol. and Food Science. 2015; 4(6):527-531. https://doi.org/10.15414/jmbfs.2015.4.6.527-531
  46. Tserkovniak LS, Bega ZT, Ostapchuk AN, Kuzmin VE, Kurdish IK. [Production of biologically active substances of indol nature by bacteria of Azotobacter Genus]. Ukr. Biochem. Z. 2009; 81(3):122-128. Russian.
  47. Chobotarov A, Volkogon M, Voytenko L, Kurdish I. Accumulation of Phytohormones by Bacteria Azotobacter vinelandii and Bacillus subtilis under Influence of Nanomaterials. J. Microbiol. Biotechn. Food Sci. 2017; 7(3):271-274. https://doi.org/10.15414/jmbfs.2017/
  48. Scorochod I, Roy A, Kurdish I. Antioxidant Potential of the Phosphate Mobilizing Bacteria Bacillus subtilis IMV B-7023 and Bacillus subtilis IB-22. J. Free Radicals and Antioxidants. 2014; 141:371-377.
  49. Skorochod IO, Kurdish IK. [Antioxidant and antiradical activities of the culture medium of Azotobacter vinelandii IMV B-7076]. Abstract of reports XV-th Congress of Vinogradskyi Society of Microbiologists of Ukraine. September 11-15, 2017. Odesa, 2017; p. 149. Ukrainian.
  50. Scorochod I, Roy A, Kurdish I. Influence of Silica Nanoparpicles on Antioxidant Potencial of Bacillus subtilis IMV B-7023. Nanoscale Res. Lett. 2016; 11:1-6.
  51. Skorochod IO, Kurdish IK. [Influence of Nanoparticles of Silica and Vermiculite on Activity of Enzymes of Antioxidant Defense] Microbiol. Biotechnol. 2013; (1):59-67. Ukrainian.
  52. Chobotarov AYu, Skorochod IO, Kurdish IK. [The influence of some nanomaterials and cations on superoxide dismutase activity of Azotobacter vinelandii IMV B-7076]. Abstract of reports XV-th Congress of Vinogradskyi Society of Microbiologists of Ukraine. September 11-15. 2017. Odesa. 2017; p. 157. Ukrainian.
  53. Skorochod IO, Tserkovniak LS, Kurdish IK. [The antioxidant effect of Bacillus subtilis and Azotobacter vinelandii on the seeds of cereals]. Microbiol. Z. 2011; 73(1):44-50. Ukrainian.
  54. Skorochod IO, Roy AO, Melentiev OI, Kurdish IK. [Influence of Bioactive Substances of Phosphate-mineralising Strains of Genus Bacillus on Plant Seeds affectted by Oxidative Stress]. Microbiology and Biotechnology. 2013; (2):41-51. Russian.
  55. Kurdish IK, Antonyk TS, Chuiko NV. Influence of Environmental Factors on the Chemotaxis of Bradyrhizobium japonicum. Microbiology. 2001; 70(1): 91-95. https://doi.org/10.1023/A:1004805207396
  56. Chuiko NV, Gordienko AS, Kurdish IK. Chemotacsis and Growth of Bradyrhizobium japonicum in Presence of Fine-Dispersed Silica. Microbiology. 2006; 75(1):44-47. https://doi.org/10.1134/S0026261706010097
  57. Chuiko NV, Kurdish IK. [The influence of high-dispersity Saponite and Silicone Dioxide on Chemotaxis of Azotobacter vinelandii SMV B-7076 and Bacillus subtilis IMV B-7023]. Microbiol. Z. 2017; 79(3):36-43. Russian. https://doi.org/10.15407/microbiolj79.03.036
  58. Kurdish IK, Chuiko NV, Bega ZT. Chemotactic and Adhesive Properties of Azotobacter vinelandii and Bacillus subtilis. Appl. Biochem. Microbiol. 2010; 46(1):51-56. https://doi.org/10.1134/S0003683810010084
  59. Chuiko NV, Gordienko AS, Kurdish IK.Chemotaxis of Azotobacter vinelandii and Bacillus subtilis in Mixed Culture. Microbiology. 2013; 82(2):186-189. https://doi.org/10.1134/S0026261713020033
  60. Kurdish IK, Bega ZT, Gordienko AS, Dyrenko DI. The Effect of Azotobacter vinelandii on Plant Seed Germination and Adhesion of these Bacteria to Cucumber Roots. Appl. Biochem. Microbiol. 2008; (4):400-404. https://doi.org/10.1134/S000368380804011X
  61. Kurdish IK, Bega ZT, Bulavenko LV, Direnko DI. [Features of adhesion of bacteria of the genus Azotobacter, strain Bacillus subtilis IMV B-7023 to the roots of plants and their colonization]. Materials of the VI International Scientific Conference (Minsk, June 2-6, 2008, Vol.2) Minsk. 2008:121-123. Russian.
  62. Declarative patent for invention 57269 A. [Method for obtaining granular bacterial preparation] Kurdish IK., Roy AO., Bega ZT. Published 18.06.2003. Bul. 6. Ukrainian.
  63. Roy AO, Zaloilo OV, Chernova LS. [Antagonistic activity of phosphate-mobilizing bacilli to phytopathogenic fungi and bacteria]. Agroecological Z. 2005; (1):50-55. Russian.
  64. Roy AA, Pasichnyk LA, Tserkovniak LS et al. [Influence of Bacteria of Bacillus subtilis on the Agent of Bacterial Cancer of Tomatoes] Mikrobiol. Z. 2012; 74(5):74-80. Russian.
  65. Roy AO, Matselyukh OV, Zubko PD, Varbanets LD, Kurdish IK. [Proteolitic activity of phosphorous mobilizing bacteria of Bacillus genus and their influence on some phytophages]. Agricultural microbiology. 2014; 20:66-73. Russian.
  66. Zubko PD, Kurdish IK. [Features of the spatial distribution of bacteria-components of the complex preparation Azogran in the root zone of cucumber]. Abstract of reports XV-th Congress of Vinogradskyi Society of Microbiologists of Ukraine. September 11-15, 2017. Odesa. 2017; p. 60. Ukrainian.
  67. Patent for invention 106135. [Method for obtaining a free-floving complex bacterial preparation for plant growing]. Kurdish IK, Roy AO. Published 20. 07. 2014. Bul. 14. Ukrainian.
  68. Melnikova N, Bulavenko L, Titova L, Kurdish I. [The influence of nitrogen-fixing and phosphate-mobilizing bacteria on the formation and functioning of the legume-rhizobia symbiosis in soybean plants]. Bulletin of the Lviv State Agricultural University. Agronomics. 6. Lviv. 2002; 145-151. Ukrainian.
  69. Kurdish IK. [Introduction of microorganisms in agroecosystems], Naukova dumka, Kyiv, 2010. Ukrainian.
  70. Kurdish IK, Chuiko NV, Bulavenko LV, Direnko DI. [Efficiency of the introduction of granular bacterial preparations into the agroecosystem of flowering plants]. Collection of the Uman Agrarian University. The basis for the formation of productivity of agricultucul crop with intensive cultivation technologies. 2008; p. 186-192. Ukrainian.
  71. Chuiko NV, Bega ZT, Bulavenko LV, Kurdish IK. [Influence of bacterial preparation of complex action on decorative plants growth]. Microbiol. Biotech. 2010; (2): 43-50. Russian.
  72. Shirokonos AM, Zwei YaP, Kurdish IK, Roy AO. The influence of granulated preparations on potat yield. Agroecological. Z. 2004; (3):24-28. Ukrainian.
  73. Kurdish I, Roy A, Chuiko N, Belogubova O, Bulavenko L, Bega Z, Dyrenko D, Chobotarjov A. [The application of granulated bacterial preparation complex action in a plant-grower]. Agroecological Z. 2008; 2:141-142. Ukrainian.
  74. Skorokhod IO, Tserkovniak LS, Kurdish IK, Plotnikov VV, Gylchuk VG, Korniychuk OV. [Influence of granulated bacterial preparation complex action on the growth and yield of barley]. Mikrobiol. Z. 2012; 74(3):23-28. Ukrainian.