Mikrobiol. Z. 2018; 80(2):3-13. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.02.003

Growth Peculiarities and Biosynthetic Activity of Solventogenic Bacteria of the Genus Clostridium

Skrotskyi S.A., Khomenko L.A., Voychuk S.I., Podgorsky V.S.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The aim of the work was to isolate natural solventogenic bacteria, to perform their screening and to study the peculiarities of the growth of the strains that actively produce butanol, acetone and organic acids on the starch-containing media. Methods: the current study was carried out with classic microbiological, physical and chemical methods, and by using fowcytometry and gas chromatography. Results and conclusions: There were isolated and studied anaerobic solventogenic bacteria (those that were able to produce butanol, acetone and ethanol). There were selected 5 strains that intensively produced solvents. These strains were identifed as Clostridium acetobutylicum. The ability of these bacteria to synthesize acetone, butanol and ethanol under growth on various starch-containing media was studied. Shown that the highest quantities of butanol (10.4 g/L and 9.0 g/L) were synthetized by the strains SS-2 and SS-5 respectively, that were, at the same time, resistant to high (cytotoxic) concentrations of butanol.

Keywords: Clostridium, biobutanol, fatty acids, biodisel, solventogenic bacteria.

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