Mikrobiol. Z. 2018; 80(1):57-66.
doi: https://doi.org/10.15407/microbiolj80.01.057

Biofilm Formation, Adhesion and Motility of Bacteria Isolated from
Children with Urinary Tract Infections

Vodianyk A.A.1, Grechukha Y.O.1, Druzenko M.O.1, Kornijko Y.Y.1, Ponyatovskiy V.A.1, Gniloskurenko A.V.1,
Lavrenchuk O.V.2, Bagdasarova I.V.2, Rudenko A.V.3, Mitiuriaeva-Kornijko I.O.1

1Bogomolets National Medical University
13 T.Shevchenko Blvd., Kyiv, 01601, Ukraine

2Institute of Nephrology, NAMS of Ukraine
17-B Degtyarevskaya Str., Kyiv, 04050, Ukraine

3Institute of Urology, NAMS of Ukraine
9-A V.Vinnichenko Str., Kyiv, 04053, Ukraine

The purpose of this research is to determine the intensity of biofilm formation, adhesion and motility of bacteria depending on different types of children’s urinary tract infections. Materials and methods: the paper contains the research of adhesion, motility and biofilm formation properties of 44 clinical isolates of bacteria from children with lower urinary tract infections, acute and chronic pyelonephritis. The ability of bacteria to form biofilms was tested using the method of microtiter plates. The motility of the microorganisms was tested using standard methods. The ability of bacteria to adhere was investigated according to Brilis, through the use of formalinized erythrocytes. Results: differences were found in the ability to form biofilm between strains of bacteria that cause infections of the lower urinary tract (optical density 0.40 ± 0.06) and chronic pyelonephritis (optical density 0.48 ± 0.07) (p < 0.05). Furthermore, a correlation was established between adhesion and biofilm formation abilities of bacteria (r = 0.529 (p < 0.01)). It was detected that increased motility ability of the bacteria reduces the force applied by this strain to form biofilm. Conclusions: bacteria isolated from children with acute and chronic pyelonephritis showed higher ability to adhere and form biofilm than the bacteria isolated from children with lower urinary tract infection. High ability to form biofilm and adhere of the isolated bacteria can be considered an adverse predictive factor in the course of urinary tract infection.

Keywords: biofilm, adhesion, urinary tract infection, bacteria adhesion.

Full text (PDF, in English)

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