Mikrobiol. Z. 2018; 80(1):45-56. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.01.045
Metabolites of Chaetomium cochliodes Palliser with Phytostimulating and Protective Activity
Drogovoz I.V.1, Kopylov I.P.2, Yovenko A.S.2
1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
2Institute of Agricultural Microbiology and Agricultural Production, NAAS of Ukraine
97 Shevchenko Str., Chernihiv, 14027, Ukraine
Arm. Perform qualitative and quantitative analysis of C. cochliodes 3250 metabolites which determine itsmold growth stimulating activity. Methods. Microbiological, physiological and physical-chemical methods were used, such as TLC spectrodensitometry, liquid chromatography-mass spectrometry. Results. We established that cultural liquid of the mold C. cochliodes 3250 stimulates winter wheatbiomass accumulation during 1:100 − 1:10000 dilution. The substancesreferred to auxins and gibberellins had been discovered by specifc biological tests. Their presence in micellium was confrmed with physical and chemical analysis. Besides cholesterol and ergosterol were detected there. The mold micellium contained 2,4-epibrassinolide in quantity of 45.71 mkg/g according to its dry weight. This substance also showed strong stimulation action. Conclusion. Three mentioned classes of phytohormonal compounds among C. cochliodes 3250 exometabolites have phytostimulating activity; 2,4-epibrassinolide plays a signifcant role in induction of plants protective reactions.
Keywords: C. cochlioides 3250, exometabolites, specific biological test, hromatographic analysis, phytohormones stimulants, biological activity.
Full text (PDF, in Ukrainian)
- Turetskaya RX. [Endogeneous factors of plant roots formation. In Biology of Plants].Moscow: Nauka; 1975. Russian.
- Hooleya R. Auxin signaling in with targeted genetics. Plant. Cell. 1998; 10:1581–1584. https://doi.org/10.1105/tpc.10.10.1581
- Dragovoz IV, Yavorska VK, Antoniuk VP, Kurchii BA. [Hormonal substances produced by microorganism association from ginseng roots]. Fiziol. and Biochem. cult. sol. 2009; 41(5):393-399. Ukrainian.
- Kopilov EP. [Soil fungi as biotic factor of influence on plants]. Agricultural microbiology. 2012; 15-16:7–28. Ukrainian.
- Shimada A., Takeuchi S., Nakajima A. Phytotoxicity of Indole-3-acetic Acid Produced by the Fungus Pythium aphanidermatum. Bioscience, Biotechnology and Biochemistry. 2000; 64(1):187–189. https://doi.org/10.1271/bbb.64.187
- Tsavkelova EA., Klimova SYu., Cherdyntseva TA., Netrusov AI. [Hormones and hormone-like substances of microorganisms: A review]. Applied Biochemistry and Microbiology. 2006; 42(3):229-235. https://doi.org/10.1134/S000368380603001X
- Janitor A. Growth of mycelia of phytopathogenic fungi after application of abscisic acid in vitro conditions. Plant Protect. Sci. 2002; 38(3):94–97. https://doi.org/10.17221/4857-PPS
- Tyuterev SL. [Ecologically safe inducers of plant resistance to diseases and physiological stresses]. Journal of plant protection. 2015; 1(83):3-13. Russian.
- Sharikova FM. [Non-specifc plant resistance to stress factors and its regulation]. Ufa: "Guillem" Publisher; 2001. 160 p. Russian.
- Biliavska LA, Galagan TA, Boltovskaya EV, Kozyritska VE, Valagurova EV, Sigereva DD, et al. [Antinematodnye properties of Streptomyces avermitilis UCM Ac-2179 and avermectin complex – averkom]. Agrarian Science. 2009; 1:29-33. Ukrainian.
- Dragovoz IV, Leonova NO, Zhukova DA, Avdeeva LV. [Exometabolites phytostimulation activity of antagonistic active stain Bacillus amyloliquefaciens IMV B-7404]. Microbiology and biotechnology. 2013; 3:84-93. Ukrainian.
- Boychuk OB Zaitsev LM [Application of short segments wheat coleoptiles test to determine the auxin]. Ukr. Botan. Zh. 1977; 6:632-636. Ukrainian.
- Kholodny Institute of Botany. [Guidelines on determination of plant hormones]. Kyiv; 1988. Russian.
- Muromtsev GS, Agnistikova VN. [Gibberellins: Monography]. Moscow: Nauka; 1984.
- Savinskiy SV, Dragovoz IV, Pedchenko VK. [Determination of indole-3-acetic acid and abscisic acid in a plant sample by HPLC]. Fiziol. and Biochem. cult. sol. 1991; 23(6):611-619. Russian.
- Iutinska GA, Ponomarenko SP, editors. [Bioregulation of microbial-plant systems: Monograph]. Kyiv: Nichlava; 2010. 472 р. Ukrainian.
- Brass D. Biological efects of brassinosteroids. Crit. Rev. Biochem. Mol. Biol. 1999; 34:339-358. https://doi.org/10.1080/10409239991209345
- Mandava NB. Plant growth-promoting brassinosteroids. Annu. Rev. Physiol. Plant Mol. Biol. 1998; 39:23-52. https://doi.org/10.1146/annurev.pp.39.060188.000323
- Dragovoz IV, Leonova NO, Biliavska L O, Yavorska VK, Iutynska GO. [Phytohormone production by some free-living and symbiotic soil microorganisms]. Reports of the National Academy of Sciences of Ukraine. 2010; 12:154-159. Ukrainian.
- Gogala N. Regulation of mycorrhizal infection by hormonal factors produced by hosts and fungi . Experientia. 1991; 47: 331-340. https://doi.org/10.1007/BF01972074
- Charvet-Candela V, Hitchin S, Ernst D, Sandermann HJr, Marmeisse R, Gay G. Characterization of an Aux/AA cDNA upregulated in Pinus pinaster roots in response to colonization by the ectomycorrhizal fungus Hebeloma cylindrosporum. New Phytologist. 2002; 154:769-777. https://doi.org/10.1046/j.1469-8137.2002.00423.x
- Reddy SM, Pandey AK, Melayah D, Marmeisse R, Gay G. The auxin responsive gene-C61 is up-regulated in Pinus pinaster roots following inoculation with ectomycorrhizal fungi. Plant, Cell and Environment. 2003; 26:681-691. https://doi.org/10.1046/j.1365-3040.2003.01003.x
- El-Zayat SA. Preliminary studies on laccase production by Chaetomium globosum an endophytic fungus in Glinus lotoides. American-Eurasian J. Agric. & Environ. Sci. 2008; 1(3):86-90.
- Ding G., Song Y.C., Chen J.R. et al. Chaetoglobosin U, a cytochalasin alkaloids from endophytic Chaetomium globosum IFB-E019. J. Nat. Prod. 2006; 69:302-304. https://doi.org/10.1021/np050515+
- Kopilov EP. Soil saprophytic fungi - natural regulators of growth, development and plant resistance to pathogen. Palmarium academic publishing, AV Akademikerverlag GmbH&Co. KG. 2013. 104 р.
- Kopilov EP. Nadkernichny SP. [The efciency of symbiotic interaction of the fungus Chaetomium cochliodes Palliser of soybean plants]. Fiziol. and Biochem. cult. sol. 2008; 40(3):260-267. Russian.
- Sakurai A, Fujioka S. The current status of physiology and biochemistry of brassinosteroids. A review. J. Plant Growth Reg. 1993; 13:147-159. https://doi.org/10.1007/BF00024257
- Bajguz A, Czerpak R. Physiological and biochemical role of brassinosteroids and their structure-activity relationship in the green alga Chlorella vulgaris Beijerink (Chlorophyceae). J. Plant Growth Reg. 1998; 17:131-139. https://doi.org/10.1007/PL00007026
- Sasse JM. Resent progress in brassinosteroid research. Physiol. Plant. 1997; 100: 696-701. https://doi.org/10.1111/j.1399-3054.1997.tb03076.x
- Pullman GS, Zhan Y, Phan BH. Brassinolide improves embryogenic tissue initiation in conifers and rice. Plant Cell Rep. 2003; 22:96-104. https://doi.org/10.1007/s00299-003-0674-x
- Franck-Duchenne M, Wang Y, Tahar SB, Beachy RN. In vitro stem elongation of sweet pepper in media containing 24-epi-brasinilide. Plant Cell Tissue and Organ Culture. 1998; 53:79-84. https://doi.org/10.1023/A:1006071803855
- Li PF, Zhou YH, Xia XJ, Shi K, Chen Z X. Brassinosteroids-induced systemic stress tolerance was associated with increased transcripts of several defence-related genes in the phloem in Cucumis sativus. PLoS ONE [Internet]. 2013 June [scholarship 2016 October]; 8(6). https://doi.org/10.1371/journal.pone.0066582
- Bekmakhanova NE., Shemshura ON. State of the problem plant resistance to fungal pathogens. Reports of National academy of sciences of the Republic of Kazakhstan. 2014; 6(6):64-69. Russian.
- Boller Th., Felix G.A. Renaissance of Elicitors: Perception of elicitors: perception microbe–associated molecular pattenis danger signals by pattern–recognition receptors. Annu. Rev. Plant Biomet. 2009; 60:379-406. https://doi.org/10.1146/annurev.arplant.57.032905.105346
- Shoresh M., Harman GE. Mastouri F. Induced Systemic Resistance and Plant Responses to Fungal Biocontrol Agents. Annu. rev. of phytopathology. 2010; 48:21-43. https://doi.org/10.1146/annurev-phyto-073009-114450
- Roatti B., Perazzolli M., Gessler C., Pertof I. Abiotic Stresses Afect Trichoderma harzianum T-39 – induced resistance to downy mildew in Grapevine. Phytopathology. The American Phytopathological Society. 2013; 103(12):1227-1234. https://doi.org/10.1094/PHYTO-02-13-0040-R
- Conrad V. Molecular aspects of defense priming. Trends in Plant Science. 2011; 16:524-531. https://doi.org/10.1016/j.tplants.2011.06.004
- Pieterse CMJ. Dicke M. Plant interaction with microbes and insects: from molecular mechanisms to ecology. Irend Plant Sci. 2007; 12:564-569. https://doi.org/10.1016/j.tplants.2007.09.004
- Katsumi M. Interaction of a brassinosteroid with IAA and GA3 in the elongation of cucumber hypocotyl sections. Plant Cell Physiol. 1985; 26: 615-625. https://doi.org/10.1093/oxfordjournals.pcp.a076950