Mikrobiol. Z. 2018; 80(1):45-56. Ukrainian.
doi: https://doi.org/10.15407/microbiolj80.01.045

Metabolites of Chaetomium cochliodes Palliser with Phytostimulating and Protective Activity

Drogovoz I.V.1, Kopylov I.P.2, Yovenko A.S.2

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Institute of Agricultural Microbiology and Agricultural Production, NAAS of Ukraine
97 Shevchenko Str., Chernihiv, 14027, Ukraine

Arm. Perform qualitative and quantitative analysis of C. cochliodes 3250 metabolites which determine itsmold growth stimulating activity. Methods. Microbiological, physiological and physical-chemical methods were used, such as TLC spectrodensitometry, liquid chromatography-mass spectrometry. Results. We established that cultural liquid of the mold C. cochliodes 3250 stimulates winter wheatbiomass accumulation during 1:100 − 1:10000 dilution. The substancesreferred to auxins and gibberellins had been discovered by specifc biological tests. Their presence in micellium was confrmed with physical and chemical analysis. Besides cholesterol and ergosterol were detected there. The mold micellium contained 2,4-epibrassinolide in quantity of 45.71 mkg/g according to its dry weight. This substance also showed strong stimulation action. Conclusion. Three mentioned classes of phytohormonal compounds among C. cochliodes 3250 exometabolites have phytostimulating activity; 2,4-epibrassinolide plays a signifcant role in induction of plants protective reactions.

Keywords: C. cochlioides 3250, exometabolites, specific biological test, hromatographic analysis, phytohormones stimulants, biological activity.

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