Mikrobiol. Z. 2018; 80(1):15-26. Russian.
doi: https://doi.org/10.15407/microbiolj80.01.015

Intensification of Acinetobacter calcoaceticus IMV B-7241 Surfactants
Synthesis on Waste Sunflower Oil

Pirog T.P.1,2, Nikituk L.V.1, Antonuk S.I.1, Shevchuk T.A.2, Iutynskaya G.A.2

1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To establish cultivation conditions of Acinetobacter calcoaceticus IMV B-7241 on waste (refried) sunflower oil of various quality, which provide the maximal indicators of surfactants synthesis. Methods. A. calcoaceticus IMV B-7241 was grown in liquid medium containing 2 − 6 % oil after frying the potato and meat including in the presence of biosynthesis precursors (glucose, organic acids). The amount of synthesized surfactants was determined by the weight method after extraction from the supernatant of culture liquid with a mixture of chloroform and methanol (2 : 1), emulsifying properties according to emulsification index of culture liquid using sunflower oil as a substrate. Results. The highest indices of surfactant synthesis on all substrates were observed with using the inoculum grown on the corresponding fried oil. The maximum concentration of surfactants (8.5 ± 0.42 and 7.9 ± 0.39 g/l) was achieved under cultivation of IMV B-7241 strain on oil after frying meat (4 %) and potato selyanski (6 %) respectively. When the biosynthesis precursors (glucose, 0.1 %, potassium fumarate, 0.01 − 0.1% and potassium citrate, 0.01 − 0.1 %) were added into medium with 2 % of the fried oil, the surfactants concentration was increased by 2.3 − 3.4 times in comparison with the indices on medium without glucose and organic acids. Addition of fumarate and citrate in form of sodium salts into the medium of IMB B-7241 strain cultivation was not accompanied by increasing surfactants synthesis due to inhibitory effect of sodium cations on activity of enzymes of anaplerotic reaction (phosphoenolpyruvate-(PEP)-carboxylase) and surfactants biosynthesis (PEP-carboxykinase, NADP+-dependent glutamate dehydrogenase). Conclusions. A doubling of waste oil and urea concentration in cultivation medium of IMV B-7241 strain (up to 4 % and 0.7 g/l, respectively) and replacing refined oil in medium for inoculum obtaining by waste was accompanied by increasing amount of synthesized surfactants in 4 − 5 folds in comparison with those on the base medium. The using fried oils for surfactants biosynthesis will allow not only to reduce the cost of final product, but also to utilize toxic waste, emissions of which into the environment in Ukraine are not regulated.

Keywords: Acinetobacter calcoaceticus IMV B-7241, surfactants, fried sunflower oil, precursors of biosynthesis, intensification of biosynthesis.

Full text (PDF, in Russian)

  1. Pleissner D, Lin C. Valorisation of food waste in biotechnological processes. Sustainable Chem. Proces. 2013; 1:21. https://doi.org/10.1186/2043-7129-1-21
  2. Baiano A. Recovery of biomolecules from food wastes − a review. Molecules. 2014; 19(9): 14821−42. https://doi.org/10.3390/molecules190914821
  3. Hossain A, AlEissa M. Biodiesel fuel production from palm, sunfower waste cooking oil and fish byproduct waste as renewable energy and environmental recycling process. British Biotechnol. J. 2016; 10(4).
  4. Panadare DC, Rathod VK. Applications of waste cooking oil other than biodiesel: a review. IJChE. 2015; 12(3): 55–76.
  5. Banat IM, Satpute SK, Cameotra SS, Patil R, Nyayanit NV. Cost efective technologies and renewable substrates for biosurfactants' production. Front. Microbiol. 2014; 5.
  6. Pirog TP, Soflkanich AP, Pokora KA, Shevchuk TA, Iutinskaya GA. [Synthesis of surfactants by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii IMV B-7405 on industrial waste]. Microbiol. Zh. 2014; 76(2): 18−24. Russian.
  7. Bordin K, Kunitake MT, Aracava KK, Trindade CS. Changes in food caused by deep fat frying − a review. Arch. Latinoam. Nutr. 2013; 63(1): 5–13.
  8. Zhang Q, Saleh AS, Chen J, Shen Q. Chemical alterations taken place during deep-fat frying based on certain reaction products: a review. Chem. Phys. Lipids. 2012; 165 (6): 662−81. https://doi.org/10.1016/j.chemphyslip.2012.07.002
  9. Pidhorskyy V, Iutinska G, Pirog T. Intensifcation of microbial synthesis technologies. Kyiv: Nauk. Dumka, 2010. 327 p. Ukrainian.
  10. Pirog TP, Shevchuk TA, Konon AD. Dolotenko EYu. Production of surfactants by Acinetobacter calcoaceticus K-4 grown on ethanol with organic acids. Appl. Biochem. Microbiol. 2012; 48(6): 569–76. https://doi.org/10.1134/S0003683812040102
  11. Pirog T, Shulyakova M, Soflkanych A, Shevchuk T, Maschenko O. Biosurfactant synthesis by Rhodococcus erytropolis IMV Ac -5017, Acinetibacter calcoaceticus IMV B-7241, Nocardia vaccinii IMV B-7405 on byproduct of biodiesel production. Food Bioprod. Proces. 2015; 93(1): 11−8. https://doi.org/10.1016/j.fbp.2013.09.003
  12. Bradford M. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976; 72(3): 248–54. https://doi.org/10.1016/0003-2697(76)90527-3
  13. Guillén MD, Uriarte PS. Aldehydes contained in edible oils of a very diferent nature after prolonged heating at frying temperature: Presence of toxic oxygenated α,β unsaturated aldehydes. Food Chem. 2012; 131(3): 915−26. https://doi.org/10.1016/j.foodchem.2011.09.079
  14. Wadekar SD, Kale SB, Lali AM, Bhowmick DN, Pratap AP. Microbial synthesis of rhamnolipids by Pseudomonas aeruginosa (ATCC 10145) on waste frying oil as low cost carbon source. Prep. Biochem. Biotechnol. 2012; 42(4): 249−66. https://doi.org/10.1080/10826068.2011.603000
  15. Oliveira JG, Cruz CHG. Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources. Braz. Arch. Biol. Technol. 2013; 56(1): 155–60. https://doi.org/10.1590/S1516-89132013000100020
  16. Zainatul AS, Yusof SZ, Asshifa MS. Fed-batch production of valuable biosurfactant, rhamnolipid, from waste cooking oil by indigenously isolate Pseudomonas aeruginosa USM-AR2. Adv. Environ. Biol. 2014; 8(14): 33–8.
  17. Pirog TP, Antonyuk SI, Konon AD, Shevchuk TA, Parfenyuk SA. [Infuence of pH on synthesis of Acinetobacter calcoaceticus IMV B-7241 biosurfactants]. Microbiol. Zh. 2013; 75(3): 40−8. Russian.
  18. Pirog TP., Konon AD. Improvement of the technology for surfactant synthesis by Acinetobacter calcoaceticus IMV B-7241. Biotechnologia acta. 2015; 8(5): 27−38.
  19. Ivanovskiy RN. Bioenergy and transport of substrates in bacteria. M.: MAKSPress, 2001, 46. Russian.