Mikrobiol. Z. 2017; 79(6):105-119.
doi: https://doi.org/10.15407/microbiolj79.06.105

Effects of Oral and Vaginal Administration of Probiotic Bacteria on the Vaginal Microbiota and
Cytokines Production in the Case of Experimental Staphylococcosis in Mice

Lazarenko L.M.1, Babenko L.P.1, Mokrozub V.V.1, Demchenko O.M.2, Bila V.V.2, Spivak M.Ya.1,3

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Perinatal Center
9 Predslavinskaya Str., Kyiv, 03150, Ukraine

35 Svitlyskoho Str., Kyiv, 04123, Ukraine

Оbjective. The objective of this study was to elucidate the efects of the probiotic strain Lactobacillus casei ІМV В-7280 and L. casei IMV B-7280 - Bifdobacterium animalis VKL - B. animalis VKB (Lc - VKL - VKB) or B. animalis VKL - B. animalis VKB (VKL - VKB) strain compositions that administrated both orally and vaginally or only orally, on the vaginal microbiota and cytokines production in experimental vaginal staphylococcosis in BALB/c mice. Methods. Probiotic bacteria administrated into staphylococcus-infected mice vaginally and orally simulntaneously or only orally. Material from the vagina was plated on selective media; the number of colony forming units was counted. The concentration of cytokines in the blood serum of mice was determined by enzyme immunoassay method. Results. It was found, that the number of aerobic and facultative anaerobic microorganisms in the vagina of staphylococcus-infected mice was decreased after administration of these probiotic bacteria or its compositions both into vagina and orally or only orally. The increase in the number of lactic acid bacteria (LAB) and bifdobacteria in the vagina of these mice was also observed. However, the number of coliform bacteria and microscopic fungi was smaller and the number of LAB was greater after administration of L. casei IMV B-7280 or Lc - VKL - VKB and VKL - VKB compositions into staphylococcus-infected mice vaginally and orally simultaneously, compared to only oral administration. A change of the vaginal microbiota of staphylococcus-infected mice that received these probiotic bacteria was accompanied with higher level of interferon-γ and interleukine-12 production, which favors the development of Th1 immune response and decrease of the production of pro-infammatory cytokine tumor necrosis factor-α. The immunomodulatory efectiveness of L. casei IMV B-7280 and Lc - VKL - VKB composition will also be higher if they are administered into staphylococcus-infected mice both orally and vaginally than only orally. Conclusions. Probiotic strain L. casei IMV B-7280 and Lc - VKL - VKB or VKL - VKB strain compositions can be a good candidate for the creation of immunobiotics for treatment of urogenital infections.

Key words: lactic acid bacteria, bifdobacteria, microbiota, vagina, staphylococci, cytokines, mice.

Full text (PDF, in English)

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