Mikrobiol. Z. 2017; 79(6):82-94. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.06.082

Symbiotic Relationship between Bradyrhizobium japonicum Strains
of Different Genetic Groups and Soybean Plants

Krutylo D.V.

Institute of Agricultural Microbiology and Agro-industrial Manufacture, NAAS of Ukraine
97 Shevchenko Str., Chernihіv, 14027, Ukraine

Aim. The aim of our work was to research the relationship between the host-plant and strains of soybean nodule bacteria, which difer in growth rates and belong to diferent genetic groups. Methods. Microbiological, serological, vegetation and feld experiments, statistical. Results. It was found, when interacting with soybean of two slow growing strains B. japonicum 46 (group USDA 6T) and B. japonicum M8 (group USDA 4), the formation of nodules by one of them led to complete blocking of nodule formation by another strain, that contacted the root to six days later. However, with the delayed inoculation of soybean by slow- (B. japonicum 46) and intensive-growing (B. japonicum KB11, group USDA 123) strains, nodules were formed with the participation of the strain that frst infected the plants, and with the participation of the second strain. The B. japonicum strain 46 formed 63.3 - 73.3 % nodules and it was more competitive than when strains of B. japonicum 46 and B. japonicum M8 was used together. With the simultaneous use of strains B. japonicum 46 and B. japonicum KB11, an insignifcant predominance of the intensive-growing strain (51.7 - 55.0 % nodules to 48.3 - 45.0 % nodules) was observed. In the feld experiment it was observed the domination in the nodules of soybean varieties of Ustya and Heihe 6 of intensive-growing nodule bacteria of the serogroup KB11 (71.6 % and 56.3 % respectively). Slow-growing rhizobia of serogroups 46 and M8 were found in a small number of nodules (2.9 - 7.4 %). The introduction of B. japonicum 46 strain increased its number in nodules to 28.6 % against the background of large heterogeneous population of rhizobia. Conclusions. Existing diferences between B. japonicum KB11, B. japonicum 46 and B. japonicum M8 strains in the organization of the genome and phenotypic properties are also manifested in their interaction with the host-plant. The intensive-growing strain of B. japonicum KB11 was most adaptable to colonization of roots and the formation of symbiosis with soybean. The determining role of macrosymbiont in regulating the number of nodules and in selective interaction with representatives of the heterogeneous population of soybean rhizobia has been confrmed.

Key words: Bradyrhizobium japonicum, delayed inoculation, competitiveness, Leonard jars, system response, soybean.

Full text (PDF, in Ukrainian)

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