Mikrobiol. Z. 2017; 79(6):71-81. Ukrainian.
Resistance of Pseudomonas aeruginosa UCМ В-1 Population to Silver Nanoparticles
at Early Stages of Biofilm Formation
Balko O.I.1, Balko O.B.1, Yaroshenko L.V.1, Skorik M.A.2, Avdeeva L.V.1
1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
2Nano Technology in Medicine Ltd
68 Antonovich Str., Kyiv, 03680, Ukraine
The aim of work was investigation of P. aeruginosa UCМ В-1 population resistance to silver nanoparticles at early stages of bioflm formation. Methods. The bioflm formation regularities were studied in steady-state system. The cultivation was conducted in meat-peptone broth, also under introduction of silver nanoparticles preparation in bactericidal concentration in 15 minutes before and after the microorganism contacted with glass surface. Visualization of the bioflm components was performed by light and scanning electron microscopy with elemental analysis. Results. Bioflm earlier structures, covered with extracellular polymer substance, were discovered after 15 minutes Pseudomonas aeruginosa UCМ В-1 cells contacted glass surface in steady-state system. At this stage of bioflm formation microorganisms were resistant to bactericidal concentration of silver nanoparticles. Survival of microorganisms in bioflm form ensured maintenance of microbial population, which gradually accumulated in bioflm form and later restored by cell transition into planktonic form. Under addition of P. aeruginosa UCМ В-1 suspension in medium with bactericidal concentration of silver nanoparticles the growth of microorganisms was registered only at 3rd day of cultivation, probably due to cell-persisters. The following recreation of bacterial population occurred according to the previously described regularities, but was delayed in time. Conclusions. The origin of bioflm earlier structures covered with extracellular polymer substance at early stages of bioflmformation conditions the resistance of P. aeruginosa UCМ В-1 population to bactericidal concentration of silver nanoparticles. Under the absence of bioflm earlier structures, recreation of bacterial population is delayed and occurred due to activation of cell-persisters.
Key words: bioflm, early stages of bioflm formation, Pseudomonas aeruginosa, silver nanoparticles, bacterial resistance.
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