Mikrobiol. Z. 2017; 79(6):41-54. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.06.041

Synthesis of Extracellular Auxins by Pathogenic for Legumes Bacteria
Belongs to the Genus Pseudomonas Under Different Conditions of Cultivation

Dankevich L.A.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. The quantity and quality composition of extracellular auxin synthesized by pathogenic for legumes bacteria belongs to the genus Pseudomonas in the absence or presence of precursor in the cultivation medium has been investigated. Materials and methods. The objects of study were isolated and collection strains are pathogenic for lupine not valid bacterial taxa − “Pseudomonas lupini” and “Pseudomonas xanthochlora” as well as collections and typical strains of pathogenic bacteria for legume bacteria belongs to genus Pseudomonas. Pathogenic properties were studied using phytopathological and microbiological methods. To determine the qualitative and quantitative composition of auxin in the culture liquid of pathogenic bacteria were performed using physico-chemical methods. Results. The diference between spectrum of synthesized auxins by pathogens for legumes representatives of the genus Pseudomonas under diferent culture conditions and its quantity has been found. It has been shown that most “P. lupini” strains and typical representatives of pathogenic for legumes species P. syringae and P. savastanoi synthesize a wide range of indole compounds. Instead, the most strains of “P. xanthochlora” and the typical strain of P. marginalis pv. marginalis 9175T synthesized mainly signifcant amounts of indole-3-acetic and indole-3-carboxylic acids. The quantity of synthesized by studied strains auxin associated with range of afected plants and the level of its aggressiveness on the host plants. Conclusions. In the absence of auxin precursor synthesis − tryptophan basic synthesis of indole compounds by most studied strains remains, indicating that the plasticity of their metabolism. The relation between the ways of interaction with the plant and the quantity and quality of synthesized auxin has been found. It has been established that the level of auxin synthesis by studied bacteria that cause disease legumes, correlated with their pathogenic properties.

Key words: phytopathogenic bacteria, “P. lupini”, “P. xanthochlora”, auxins, indole-3-acetic acid.

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