Mikrobiol. Z. 2017; 79(5):80-90. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.05.080

Physiological and Biochemical Characteristics of Desulfuromusa sp. CB30,
Isolated from Coal Pits Waste Heaps

Diakiv S.V., Hnatush S.O., Halushka А.А.

Ivan Franko National University of Lviv
4 Hrushevskyi Str., Lviv, 79005, Ukraine

The study of physiological and biochemical characteristics of sulfur reducing bacteria Desulfuromusa sp. SV30, isolated from coal pits waste heaps of Chervonograd minig region was conducted. It was established that fumarate applying into Postgate C medium for cultivation of investigated bacteria caused their biomass intensifying accumulation (in 1.56 times) and hydrogen sulfde (in 1.23 times) compared with the control. Bacteria Desulfuromusa sp. SV30 utilize fumarate simultaneously as electron donor, accumulating acetate (till 3.47 g/l) in the culture fuid, as well as acceptor, reducing fumarate to succinate (4.07 g/l). Under conditions of sodium lactate and fumarate applying occurs the fumarate reducing the same as its usage as an additional carbon source. Sodium lactate is utilized after fumarate ending. Bacteria have a high rate of sulfur reductase enzyme activity (55.6 ± 9.67 µM H2S/min×mg protein, in рН range 7.5 – 9.0 and under the 20 – 40 ºС) and sulfur reductase affnity to the substrate (Km = 0.09 ± 0.02 µM).

Key words: Desulfuromusa, sulfur respiration, fumarate respiration, sulfur reductase enzyme activity.

Full text (PDF, in Ukrainian)

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