Mikrobiol. Z. 2017; 79(5):57-69. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.05.057

Gibberellins of Endophytic and Saprotrophic Penicillium funiculosum Strains

Yurieva О.M.1, Dragovoz I.V.1, Leonova N.O.1, Ostapchyk A.M.2,
Kharkhota M.A.1, Syrchin S.O.1, Kurchenko I.M.1

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Odesa Mechnikov National University
2 Dvoryanska Str., Odesa, 65082, Ukraine

Aim. Study of the general phytostimulating and gibberellic activities, as well as qualitative and quantitative composition of extracellular gibberellins of endophytic and saprotrophic Penicillium funiculosum strains. Methods. Microbiological, physiological and physico-chemical (HPLC/MS) research methods. Results. It was established that the phytostimulating activity of endophytic P. funiculosum strain was clearly indicated in undiluted culture filtrate (CF) and in 1 : 100 - 1 : 500 dilutions, whereas for saprotroph it was significantly lower and was observed only at certain dilutions (1 : 400 and 1 : 500). In specific gibberellic biotest the CF extract of endophytic strain was three times more active than saprotrophic one – 30 % and 10 %, respectively. HPLC/MS analysis showed that endophytic and saprotrophic P. funiculosum strains synthesize gibberellins: GA3 (6.8 ng/g dw and 4.1 ng/g dw, respectively), GA4 (4.9 ng/g dw and 11.6 ng/g dw) and trace amounts of GA7. Conclusions. The studied endophytic and saprotrophic P. funiculosum strains were able to synthesize biologically active gibberellins GA3, GA4 and GA7, that may play an important physiological role in the formation of symbiotic and associative relationships with plants.

Key words: Penicillium funiculosum, endophyte, saprotroph, gibberellin, biotesting, HPLC/MS.

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