Mikrobiol. Z. 2017; 79(4):75-87. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.04.075

Identification of Heterotrophic Bacteria Isolated from Soil Ferrosphere and
their Sensitivity to the Pesticide Linuron

Tkachuk N.V.1, Zelena L.B.2, Parminska V.S.1, Yanchenko V.O.1, Demchenko A.M.1

1Chernihiv National Pedagogical University named after Taras Shevchenko
53 Getman Polubotko Str., Chernihiv, 14013, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Three strains of heterotrophic ammonifying and iron-reducing bacteria were isolated from soil ferrosphere. They were identifed as Bacillus simplex, Streptomyces gardneri and Fictibacillus sp. by the complex of microbiological features, physiological and biochemical properties and on the basis of 16S rRNA gene sequences (phylogenetic analysis). The 16S rRNA gene sequences were registered in GenBank. The sensitivity of isolated bacterial strains to urea derivatives based on the pesticide linuron was studied. It was determined that B. simplex ChNPU F1 and S. gardneri ChNPU F3 strains were characterized by the high sensitivity to these derivatives but Fictibacillus sp. ChNPU ZVB1 – low one. It was defned that the derivative containing antipyrine fragment can be the perspective compound with antibacterial characteristics for protecting against microbial corrosion. It was determined the possibility to decrease linuron toxicity against soil bacteria by insertion of piperidine fragment into its molecule.

Key words: ferrosphere, heterotrophic ammonifying bacteria, heterotrophic iron-reducing bacteria, phenotypic characteristics, 16S rRNA gene, bactericides, pesticide linuron.

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