Mikrobiol. Z. 2017; 79(4):21-29. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.04.021

The Role of Acinetobacter calcoaceticus IMV В-7241 Surfactants in Biofilms Destruction

Pirog T.P.1,2, Savenko I.V.1, Lutsai D.A.1, Shevchuk T.A.2, Iutynska G.O.2

1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To study the ability of surface-active agents (surfactants) synthesized Acinetobacter calcoaceticus IMV B-7241 on various substrates (ethanol, glycerol, n-hexadecane), destroy a bioflm certain bacteria and yeasts. Methods. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). The degree of bioflm destruction was determined as difference between the number of adhered cells in untreated and treated with surfactant holes of polystyrene immunological plate containing pre-formed bioflm of test cultures and was expressed as a percentage. The number of adherent cells was determined by spectrophotometrically. Results. The dependence of degree of bioflms destruction in the presence of A. calcoaceticus IMV B-7241 surfactants on the nature of carbon source in medium of producer cultivation, concentration of surfactants in the preparations and test culture type was established. Degree of Escherichia coli ІЕМ-1 bioflm destruction in the presence of surfactants synthesized on all tested substrates practically did not differ and was 25 − 53 % depending on the concentration of the surfactants (0,04 − 1,28 mg/ml). Bacillus subtilis BT-2 bioflm destruction reached 71 − 86 % after addition of surfactants (0,16 − 0,64 mg/ml) obtained on ethanol and glycerol. Maximum destruction of Staphylococcus aureus BMS-1 bioflm (69 − 88 %) and Candida albicans D-6 (42 %) was observed in the presence of surfactants (0,32 − 1,28 mg/ml) synthesized on n-hexadecane. Conclusions. The effective concentrations of A. calcoaceticus IMV B-7241 surfactants, providing a high degree of bacterial bioflms destruction are comparable to those of the known microbial surfactants. The obtained data indicates the need for research the infuence of producer cultivation conditions on the biological properties of fnal product.

Key words: Acinetobacter calcoaceticus ІМV В-7241, surfactants, destruction of the bioflm, cultivation conditionsa.

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