Mikrobiol. Z. 2017; 79(4):12-20. Russian.
doi: https://doi.org/10.15407/microbiolj79.04.012

Influence of the Cultivation Conditions of Bacillus subtilis IMV B-7023 and
its Streptomycinresistant Mutant on the Surface Properties of these Bacteria

Roy A.A.1, Kurdish I.K.1, Ostapyuk S.N.2, Savelyev Yu.V.2

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Institute of Macromolecular Chemistry, NAS of Ukraine
48 Kharkiv Highway, Kyiv, 02160, Ukraine

Aim. It was to study the infuence of the cultivation conditions of Bacillus subtilis IMV B-7023 and its streptomycin resistant mutant in medium with calcium glycerophosphate on surface characteristics of cells by the method Fourier Transform Infrared Spectroscopy. Methods. In this experimental work was used microbiological, chemical, physical, biochemical, and statistical methods. Bacteria were grown in a mineral medium with calcium glycerophosphate as phosphorus and carbon source simultaneously with or calcium glycerophosphate and glucose. Spectra characteristic components of the cell surface of Bacillus subtilis IMV B-7023 and its streptomycin resistant mutant were recorded by the method Fourier Transform Infrared Spectroscopy on the device FT-IR Spectrometer “Tensor-37” from Bruker. Results. The cultivation conditions of bacteria in medium with calcium glycerophosphate and various sources of carbon nutrition signifcantly affected the properties of their cell surface. FT-IR spectra of the surface of cells characterize redistribution of intensity peaks mainly responsible for amide group (protein) NH-CO and an ether bond C-O and groups P-O and P-O-C depending on the use of a source of carbon nutrition by bacteria. Conclusion. The obtained results showed the possibility of utilization of glycerophosphate not only on the cell surface, as was the case of its use bacteria as a source of phosphorus (where the main role played phosphatase associated with surface structures bacilli), but also about the participation of other enzymes capable of degrading the compound at the place of carbon bonds in the molecule, it can be used as a source of carbon and phosphorus simultaneously.

Key words: Bacillus subtilis IMV B-7023, streptomycin resistant mutant, bacterial cell surface, Fournier Transform Infrared Spectra.

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