Mikrobiol. Z. 2017; 79(4):3-11. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.04.003

Influence of Physico-Chemical Factors on Dehydrogenase Activity of Azotobacter vinelandii

Kurdish I., Gerasimenko I.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. Different factors were infuence on physiology-biochemical activity of bacteria – components microbial preparations for plant-growth. The aim of this work was to study the infuence of different factors on dehydrogenase activity of Azotobacter vinelandii IMV B-7076 – compound of the complex bacterial preparation Azogran. Methods. In this experimental work was used microbiological, biochemical, and statistical methods. Dehydrogenase activity was measured accordingly to the method is based on the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) to red-colored triphenyl formazan (TF) in anaerobic conditions. Results. It was established, that the dehydrogenase activity of Azotobacter vinelandii IMV B-7076 reached its maximum value at cultivation in nutrient medium with 10 g/L of glucose, pH 9.0 and 28 °C. It was shown, that at addition of the certain concentration of ions Ca2+ and Mg2+ into a nutrient medium the dehydrogenase activity of bacteria increased. However, ions Fe2+ inhibited this activity. Conclusion. It has been determined depending of dehydrogenase activity of Azotobacter vinelandii IMV B-7076 from different physico-chemical factors. It over under standing about properties this bacteria and help predict prognoses the perceptivity use of preparation Azogran in different agroecosystems.

Key words: Azotobacter vinelandii IMV B-7076, dehydrogenase activity, physico-chemical factors.

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