Mikrobiol. Z. 2017; 79(3):125-136.
Detection of Soybean Mosaic Virus in Some Left-Bank Forest-Steppe Regions of Ukraine
Mishchenko L.T.1, Dunich A.A.1, Shevchenko T.P.1, Budzanivska I.G.1,
Polischuk V.P.1, Andryichuk O.M.1, Molchanets O.V.1, Antipov I.O.2
1Taras Shevchenko National University of Kyiv
64/13 Volodymyrska Str., Kyiv, 01601, Ukraine
2National University of Life and Environmental Sciences of Ukraine
15 Heroiv Oborony Str., Kyiv, 03041, Ukraine
Soybean (Glycine max L.) is strategic legumes world crop of XXI century, which is located in the center of attention of world agricultural science and production. Soybean mosaic virus (SMV) is the most prevalent virus and is recognized as the most serious, long-standing problem in many soybean producing areas in the world. Infection by SMV usually results in severe yield losses, seed quality, seedling viability reduction of soybean. Study of soybean viruses in Ukraine were conducted exclusively on the Right Bank of its parts. However, studies of possible spreading and circulation of these viruses in a left-bank forest-steppe has not yet been carried out. The aim of the study was to diagnose of viruses that infect soybeans in the territory of left-bank Ukraine, and investigate some of their properties. Methods: visual diagnostics, transmission electron microscopy, ELISA, RNA extraction from plant material, PCR, sequencing, phylogenetic analysis. Results. It was studied 29 samples of soybean plants in the territory of left-bank forest-steppe of Ukraine (Poltava, Sumy and Kharkiv region) on the presence of a viral infection. ELISA revealed that 48% of the samples (13 samples from the Poltava region and 1 - from Sumy) affected with Soybean mosaic virus. This is the frst report about infecting soybean grown in a left-bank of Ukraine with SMV. On the basis of previously submitted data on circulation in the territory of the right bank of the country mixed infection of this virus with Bean yellow mosaic virus (BYMV) and Alfalfa mosaic virus (AMV), the test samples were checked on the presence of these viruses. Results of the analysis showed the absence of BYMV and AMV antigens. It was found that the studied SMV isolates differ in virions size from the previously identifed Ukrainian SMV isolates. Phylogenetic analysis of the nucleotide sequence of the capsid protein gene of Soybean mosaic virus showed a 100% level of phylogenetic relatedness between the Ukrainian representative isolate and Chinese, Iranian isolates, American isolate 452, and Polish isolate M, which testifes to their common origin.
Key words: Glycine max, Soybean mosaic virus, Bean yellow mosaic virus, Alfalfa mosaic virus, ELISA, PCR, sequencing, viruses morphology, phylogenetic analysis.
Full text (PDF, in English)
- Clinton GP. Reports of the botanist for 1915: Soybeans. Annual Report, Connecticut Agricultural Experiment Station. 1916: 446.
- Gardner MW, Kendrick JB. Soybean mosaic virus. Journal of Agricultural Research. 1921; 22:111-14.
- Bilyk LG. [Soybean mosaic in Ukraine]. Thesis PhD. Kyiv; 1967. Ukrainian.
- Kyrychenko AM, Kraeva GV, Kovalenko OG. Biological characteristic and identifcation of soybean virus isolated from different Ukraine regions. Mikrobiol. Zh. 2012; 74(1):46-50.
- Sherepitko DV, Budzanivska IG, Polischuk VP, Boyko AL. Sequencing and phylogenetic analysis of Soybean mosaic virus isolated in Ukraine. Biopolym. Cell. 2011; 27(6):472–79. https://doi.org/10.7124/bc.00011A
- Wang A. Soybean mosaic virus: research progress and future perspectives. In: Proceedings of VIII World Soybean Research Conference; 2009 Ast 10-15; Beijing, China.
- Arif M, Hassan S. Evaluation of resistance in soybean germplasm to Soybean mosaic potyvirus under feld conditions. Online Journal of Biological Sciences. 2002; 2:601-604. https://doi.org/10.3923/jbs.2002.601.604
- Liao L, Chen P, Buss GR, Yang Q, Tolin SA. Inheritance and allelism of resistance to Soybean mosaic virus in Zao18 soybean from China. Buss Journal of Heredity. 2002; 93(6):447-52. https://doi.org/10.1093/jhered/93.6.447
- El-Amretz AA, El-Said HM, Salem DE. Effect of Soybean mosaic virus infection on quality of soybean seed. Agricultural Research Review. 1987; 63:155-64.
- Sherepitko DV. Molecular-genetic and biological properties of viruses (soybean mosaic potyvirus, alfalfa mosaic alfamovirus) identifed on soybean under environmental conditions of Forest-steppe zone of Ukraine. Thesis PhD. Kyiv; 2012. Ukrainian.
- Peresypkin VF, Markov IL, Shelestova VS. [Workshop on the basics of research in plant protection]. К; 2000. Ukrainian.
- Salyga YuT, Snitynsky VV. Elektronna mikroskopiya biologichnyh obyektiv. Кyiv: Svit; 1999. Ukrainian.
- Crowther JR. ELISA. Theory and practice. New York: Hamana Press; 1995.
- Melnichuk MD, Antipov IO, Spiridonov VG. Molekulyarna diagnostyla ta identyfkatcia X-, Y-, M-, S-, L- virusiv kartopli (Solanum tuberosum L.) metodom polimeraznoyi lantcyugovoyi reaktcii. Metodychni rekomendatcii. Кyiv: Publish center NAU; 2008. Ukrainian.
- Lakin GF. [Biometry]. M: High school; 1980. Russian.
- Zhou G-C, Shao Z-Q, Ma F-F, Wu P, Wu X-Y, Xie Z-Y, et al. The evolution of soybean mosaic virus: An updated analysis by obtaining 18 new genomic sequences of Chinese strains/isolates. Virus Res. 2015; 208:189-98. https://doi.org/10.1016/j.virusres.2015.06.011
- Domier LL, Latorre IJ, Steinlage TA, McCoppin N, Hartman GL. Variability and transmission of Aphis glycines of North American and Asian soybean mosaic virus isolates. Arch Virol. 2003; 148:1925-41. https://doi.org/10.1007/s00705-003-0147-0
- Jezewska M, Trzmiel K, Zarzyn´ska-Nowak A, Lewandowska M. Identifcation of Soybean mosaic virus in Poland. Journal of Plant Pathology. 2015; 97(2): 357-62.
- Ahangaran A, Habibi MK, Mohammadi GH, Winter S, Garcia-Arenal F. Analysis of Soybean mosaic virus genetic diversity in Iran allows the characterization of a new mutation resulting in overcoming Rsv4-resistance. Journal of General Virology. 2013; 94: 2557-68. https://doi.org/10.1099/vir.0.055434-0
- Seo JK, Ohshima K, Lee HG, Son M, Choi HS, Lee SH, et al. Molecular variability and genetic structure of the population of Soybean mosaic virus based on the analysis of complete genome sequence. Virology. 2009b; 393: 91-103. https://doi.org/10.1016/j.virol.2009.07.007
- Gagarinova AG, Babu M, Poysa V, Hill JH, Wang A. Identifcation and molecular characterization of two naturally occurring Soybean mosaic virus isolates that are closely related but differ in their ability to overcome Rsv4 resistance. Virus Research. 2008; 138: 50-56. https://doi.org/10.1016/j.virusres.2008.08.010
- Chowda-Reddy RV, Sun H, Chen H, Poysa V, Lin H, Gijzen M, et al. Mutations in the P3 protein of Soybean mosaic virus G2 isolates determine virulence on Rsv4-genotype soybean. Molecular Plant Microbe Interaction. 2011; 24: 37-43. https://doi.org/10.1094/MPMI-07-10-0158
- Seo JK, Lee HG, Choi HS, Lee SH, Kim KH. Infectious in vivo transcripts from a full-length clone of Soybean mosaic virus strain G5H. Plant Pathol J. 2009; 25:54-61. https://doi.org/10.5423/PPJ.2009.25.1.054
- Chen J, Zheng H, Lin L, Adams MJ, Antoniw JF, Zhao MF, et al. A virus related to Soybean mosaic virus from Pinellia ternata in China and its comparison with local soybean SMV isolates. Archives of Virology. 2004; 149: 349-63. https://doi.org/10.1007/s00705-003-0184-8
- Shi YH, Hong XY, Chen J, Adams MJ, Zheng HY, Lin L, et al. Further molecular characterisation of potyviruses infecting aroid plants for medicinal use in China. Arch. Virol. 2005; 150(1):125-35. https://doi.org/10.1007/s00705-004-0390-z
- Gibbs AJ, Trueman JW, Gibbs MJ. The Bean common mosaic virus lineage of potyviruses: where did it arise and when? Arch. Virol. 2008; 153:2177–87. https://doi.org/10.1007/s00705-008-0256-x