Mikrobiol. Z. 2017; 79(3):125-136.
doi: https://doi.org/10.15407/microbiolj79.03.125

Detection of Soybean Mosaic Virus in Some Left-Bank Forest-Steppe Regions of Ukraine

Mishchenko L.T.1, Dunich A.A.1, Shevchenko T.P.1, Budzanivska I.G.1,
Polischuk V.P.1, Andryichuk O.M.1, Molchanets O.V.1, Antipov I.O.2

1Taras Shevchenko National University of Kyiv
64/13 Volodymyrska Str., Kyiv, 01601, Ukraine

2National University of Life and Environmental Sciences of Ukraine
15 Heroiv Oborony Str., Kyiv, 03041, Ukraine

Soybean (Glycine max L.) is strategic legumes world crop of XXI century, which is located in the center of attention of world agricultural science and production. Soybean mosaic virus (SMV) is the most prevalent virus and is recognized as the most serious, long-standing problem in many soybean producing areas in the world. Infection by SMV usually results in severe yield losses, seed quality, seedling viability reduction of soybean. Study of soybean viruses in Ukraine were conducted exclusively on the Right Bank of its parts. However, studies of possible spreading and circulation of these viruses in a left-bank forest-steppe has not yet been carried out. The aim of the study was to diagnose of viruses that infect soybeans in the territory of left-bank Ukraine, and investigate some of their properties. Methods: visual diagnostics, transmission electron microscopy, ELISA, RNA extraction from plant material, PCR, sequencing, phylogenetic analysis. Results. It was studied 29 samples of soybean plants in the territory of left-bank forest-steppe of Ukraine (Poltava, Sumy and Kharkiv region) on the presence of a viral infection. ELISA revealed that 48% of the samples (13 samples from the Poltava region and 1 - from Sumy) affected with Soybean mosaic virus. This is the frst report about infecting soybean grown in a left-bank of Ukraine with SMV. On the basis of previously submitted data on circulation in the territory of the right bank of the country mixed infection of this virus with Bean yellow mosaic virus (BYMV) and Alfalfa mosaic virus (AMV), the test samples were checked on the presence of these viruses. Results of the analysis showed the absence of BYMV and AMV antigens. It was found that the studied SMV isolates differ in virions size from the previously identifed Ukrainian SMV isolates. Phylogenetic analysis of the nucleotide sequence of the capsid protein gene of Soybean mosaic virus showed a 100% level of phylogenetic relatedness between the Ukrainian representative isolate and Chinese, Iranian isolates, American isolate 452, and Polish isolate M, which testifes to their common origin.

Key words: Glycine max, Soybean mosaic virus, Bean yellow mosaic virus, Alfalfa mosaic virus, ELISA, PCR, sequencing, viruses morphology, phylogenetic analysis.

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