Mikrobiol. Z. 2017; 79(3):72-83. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.03.072
Composition Optimization of the Nutrient Medium by Plakett-Berman Design for Increasing the Enzyme Activity
of Cellulolytic Complex of Fennellia sp. 2806
Syrchin S.O., Pavlychenko А.К., Kharkevych O.S., Nakonechna L.T., Yurieva O.М., Kurchenko I.M.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. Optimization of the nutrient medium components of ascomycete Fennellia sp. 2806 to increase the synthesis of cellulolytic enzyme complex using Plackett-Burman factorial design; study of the possibility of using this method to increase the synthesis of components of cellulolytic multienzyme complex. Methods. Methods of statistical design and determination of exo-, endoglucanase and xylanase activities by determining the release of reducing sugars were used. Results. Composition optimization of the nutrient medium increased the enzyme activities of cellulolytic complex of Fennellia sp. 2806: endoglucanase – 1.2 times, exoglucanase – 2.2, xylanase – 2.4 compared to the initial medium. The signifcant factors that had the greatest impact on each of the investigated activities were determined: urea, of KH2PO4, wheat straw, KCl, CoCl2. Conclusions. Plackett-Burman design was successfully employed for the composition optimization of the nutrient medium to increase the synthesis of multienzyme cellulolytic complex of Fennellia sp. 2806.
Key words: endoglucanase, exoglucanase, xylanase, Fennellia sp, Plackett-Burman design.
Full text (PDF, in Ukrainian)
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