Mikrobiol. Z. 2017; 79(3):56-64. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.03.056

Fatty Acid Composition of Cellular Lipids Pseudomonas syringae,
Isolated from Cereal Agrophytocenosis

Butsenko L.M., Savenko O.A., Pasichnyk L.A., Shcherbyna T.M., Patyka V.P.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Identifcation of pathogenic bacteria makes it possible not only to establish the cause of the disease, but also to determine the ways of distribution of the pathogen, to study its biological characteristics and to undertake measures for protection of plants. Studies of cellular fatty acid composition of bacterial lipids of Pseudomonas genus, carried out by many authors, have shown the importance of this feature for the identifcation of this group of bacteria. Aim. To study composition of cellular lipids of Pseudomonas syringae strains, which were isolated from affected bacteriosis plant rye, wheat and segetal vegetation in crops of wheat. Methods. Work performed by classical microbiological and biochemical methods. To obtain fatty acid methyl esters bacterial cells, which grew on the PA (potato agar), was suspended in methanol, which contained 1.5 % of sulfuric acid. Analysis of fatty acid composition performed using gas chromatography-mass spectrometry system Agilent 6890N/5973 inert. Results. Investigated fatty acid profles of the 33 strains of pathogenic bacteria P. syringae species, which have been isolated from rye, wheat and weeds in wheats feld. It is found that all the strains have similar fatty acid profles. As identifed dominant fatty acids with pair number of carbon atoms - hexadecanoic (C16:0), hexadecenoic (C16:1) and octadecenoic (C18:1). As minor components detected dodecanoic, tetradecanoic, octadecanoic acids. All strains  have identifed a hydroxy fatty acids: 3-hydroxydecanoic (3-OH C10:0), 2-hydroxydodecanoic (2-OH C12:0), 3-hydroxydodecanoic (3-OH C12:0) acids. Conclusions. Fatty acid composition of cellular lipids of P. syringae strains, which were isolated from the affected bacteriosis plant rye, wheat and segetal plants in wheat feld, is similar to the form corresponds to the composition of fatty acids P. syringae. It was found that the existence of differences in the fatty acid spectrum is characteristic of each strain and no correlates with the division on pathovars and serogroup.

Key words: P. syringae, fatty acids, wheat agrocenosis, segetal plants.

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