Mikrobiol. Z. 2017; 79(3):44-55. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.03.044

Phylogenetic Analysis of Pathogenic Bacteria Belongs to the Erwinia Genus –
Agent of Apple’s Diseases in Ukraine

Dankevich L.A.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To determine the similarity of 16S pPHK gene nucleotide sequences of 10 isolated Erwinia sp. and 9 collection «Erwinia horticola» strains with typical representatives of the genus Erwinia for their correct identifcation at the species level. Methods. Objects − 10 isolated from diseased tissue of apple Erwinia sp. and 9 collection «Erwinia horticola» strains. DNA copy of the gene 16S pPHK amplifed using universal primers pA and pH. Building a reverse compliment, editing, alignment and matching sequences was performed using Multalin and Sequence Manipulation Suite software. The nucleotide similar sequences of 16S rRNA gene, that placed and for deposited in GenBank, searching was performed using the BLASTN program. The phylogenetic trees constructing was performed using MEGA 5 program. Results. Nucleotide sequence analysis of 16S rRNA gene found a high level of homology (99 - 98 %) of the test strains with typical strains of E. amylovora, E. pyrifoliae, E. piriforinigrans, E. uzenensis, E. billingiae, E. persicina, E. aphidicola species. Conclusions. Considering examined previously the complex of phenotypic properties and determined homology of 16S pPHK gene nucleotide sequences can be argued that most likely similarity have isolated Erwinia sp. and collection «Erwinia horticola» strains with typical E. amylovora and E. pyrifoliae strains. Accoding to the literature, these species are closely related and poorly differentiated based on the homology of the 16S pPHK gene nucleotide sequences. Probably for the final identifcation at the species level the strains, studied by us, and belongs to the Erwinia genus it is necessary to involvement other genotypic properties.

Key words: Erwinia sp.,  «Erwinia horticola», Erwinia amylovora, the nucleotide sequence of the gene 16S pPHK, phylogenetic analysis.

Full text (PDF, in Ukrainian)

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